Typical Testing Data/Standard Curve
Human Serine/threonine protein kinase 40 (STK40) ELISA Kit | STK40 elisa kit
Human Serine/threonine protein kinase 40 (STK40) ELISA Kit
Gene Names
STK40; SHIK; SgK495
Reactivity
Human
Synonyms
Serine/threonine protein kinase 40 (STK40); Human Serine/threonine protein kinase 40 (STK40) ELISA Kit; STK40 elisa kit
Reactivity
Human
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
2.5-50ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Related Product Information for STK40 elisa kit
Intended Uses: This STK40 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human STK40. This ELISA kit for research use only!
Principle of the Assay||STK40 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-STK40 antibody and an STK40-HRP conjugate. The assay sample and buffer are incubated together with STK40-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the STK40 concentration since STK40 from samples and STK40-HRP conjugate compete for the anti-STK40 antibody binding site. Since the number of sites is limited, as more sites are occupied by STK40 from the sample, fewer sites are left to bind STK40-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The STK40 concentration in each sample is interpolated from this standard curve.
Principle of the Assay||STK40 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-STK40 antibody and an STK40-HRP conjugate. The assay sample and buffer are incubated together with STK40-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the STK40 concentration since STK40 from samples and STK40-HRP conjugate compete for the anti-STK40 antibody binding site. Since the number of sites is limited, as more sites are occupied by STK40 from the sample, fewer sites are left to bind STK40-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The STK40 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for STK40 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
49,459 Da
NCBI Official Full Name
serine/threonine-protein kinase 40 isoform 1
NCBI Official Synonym Full Names
serine/threonine kinase 40
NCBI Official Symbol
STK40
NCBI Official Synonym Symbols
SHIK; SgK495
NCBI Protein Information
serine/threonine-protein kinase 40; sugen kinase 495; Ser/Thr-like kinase; SINK-homologous serine/threonine-protein kinase
UniProt Protein Name
Serine/threonine-protein kinase 40
Protein Family
UniProt Gene Name
STK40
UniProt Synonym Gene Names
SGK495; SHIK; SgK495
UniProt Entry Name
STK40_HUMAN
Uniprot Description
SHIK: an ubiquitously expressed protein kinase of the CAMK family. Cytoplasmic. May be a negative regulator of NF-kappaB- and p53-mediated gene transcription.
Protein type: EC 2.7.11.1; Protein kinase, CAMK; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CAMK group; CAMK-Unique family
Chromosomal Location of Human Ortholog: 1p34.3
Cellular Component: nucleoplasm; cytoplasm
Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding
Biological Process: glycogen metabolic process; respiratory system process; regulation of gene expression; regulation of MAPKKK cascade; multicellular organism growth; alveolus development; protein amino acid phosphorylation; negative regulation of apoptosis
Research Articles on STK40
Similar Products
Product Notes
The Human STK40 stk40 (Catalog #AAA7231740) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7231740 ELISA Kit recognizes Human STK40. It is sometimes possible for the material contained within the vial of "Serine/threonine protein kinase 40 (STK40), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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