Rabbit STEAP3 Polyclonal Antibody | anti-STEAP3 antibody

STEAP3 Antibody

Cat. Num. AAA9606782

• Gene Names: STEAP3; STMP3; TSAP6; pHyde; AHMIO2; dudlin-2; dudulin-2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: STEAP3; Polyclonal Antibody; STEAP3 Antibody; 1010001D01Rik; Dudlin 2; Dudlin2; Dudulin 2; Dudulin-2; Dudulin2; hpHyde; hTSAP6; Metalloreductase STEAP3; MGC93147; pHyde; Six transmembrane epithelial antigen of prostate 3; Six transmembrane prostate protein 3; Six-transmembrane epithelial antigen of prostate 3; STEA 3; STEA3_HUMAN; STEAP 3; STEAP family member 3; STEAP family member 3 metalloreductase; STMP 3; STMP3; TSAP 6; TSAP6; Tumor suppressor activated pathway protein 6; Tumor suppressor pHyde; Tumor suppressor-activated pathway protein 6; anti-STEAP3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: STEAP3 antibody detects endogenous levels of total STEAP3
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 488

• Applicable Applications for anti-STEAP3 antibody: Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF/ICC: 1:100-1:500
• Immunogen: A synthesized peptide derived from human STEAP3
• Subcellular Location: Endosome Membrane. Localizes to vesicular-like structures at the plasma membrane and around the nucleus.
• Tissue Specificity: Expressed in adult bone marrow, placenta, liver, skeletal muscle and pancreas. Down-regulated in hepatocellular carcinoma.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of Mouse liver tissue lysates, using STEAP3 Antibody. The lane on the left is treated with the antigen-specific peptide.)

Immunofluorescene (IF)

(MBS9606782 staining COLO205 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

Immunohistochemistry (IHC)

(MBS9606782 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Related Product Information for anti-STEAP3 antibody

Description: Caspases modulate apoptosis in various ways. Specifically, Caspase-3, a death protease, is instrumental in cleaving cellular proteins, dismantling the cell and forming apoptotic bodies. pHyde has a potential role as a tumor suppressor by inducing caspase-3-mediated apoptosis and stimulating p53 expression. A dose-dependent increase in caspase-3 activity is observed in transduced pHyde DU145 cells. Furthermore, caspase-3 may be necessary for pHyde-mediated apoptosis. The pHyde gene may upregulate the apoptosis pathway and thus have a potential application in cancer gene therapy. Recombinant pHyde inhibits the growth of human prostate cancer cell lines DU145 and LNCaP in vitro. DU145 tumors may be reduced significantly in vivo when nude mice are injected with recombinant pHyde. pHyde also has a demonstrated growth inhibitory effect on human breast cancer cells. This suggests that pHyde may have a role in inhibiting different tumor types.
Function: Endosomal ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells. Participates in erythroid iron homeostasis by reducing Fe3+ to Fe2+. Can also reduce of Cu2+ to Cu1+, suggesting that it participates in copper homeostasis. Uses NADP+ as acceptor. May play a role downstream of p53/TP53 to interface apoptosis and cell cycle progression. Indirectly involved in exosome secretion by facilitating the secretion of proteins such as TCTP.
Subunit Structure: Homodimer. Interacts with BNIP3L, MYT1, RHBDL4/RHBDD1 and TCTP.
Post-translational Modifications: Proteolytically cleaved by RHBDL4/RHBDD1. RHBDL4/RHBDD1-induced cleavage occurs at multiple sites in a glycosylation-independent manner. Glycosylated.
Similarity: Belongs to the STEAP family.

NCBI and Uniprot Product Information

• NCBI GI #: 56549147
• NCBI GeneID: 55240
• NCBI Accession #: NP_001008410.1
• NCBI GenBank Nucleotide #: NM_001008410.1
• UniProt Accession #: Q658P3; Q4VBR2; Q4ZG36; Q53SQ8; Q7Z389; Q86SF6; Q8NEW6; Q8TDP3; Q8TF03; Q9NVB5; A8K6E3
• Molecular Weight: Observed: 55 kDa; Predicted: 55 kDa
• NCBI Official Full Name: metalloreductase STEAP3 isoform b
• NCBI Official Synonym Full Names: STEAP3 metalloreductase
• NCBI Official Symbol: STEAP3
• NCBI Official Synonym Symbols: STMP3; TSAP6; pHyde; AHMIO2; dudlin-2; dudulin-2
• NCBI Protein Information: metalloreductase STEAP3
• UniProt Protein Name: Metalloreductase STEAP3
• Protein Family: Metalloreductase
• UniProt Gene Name: STEAP3
• UniProt Synonym Gene Names: TSAP6; hTSAP6; hpHyde

NCBI Description

This gene encodes a multipass membrane protein that functions as an iron transporter. The encoded protein can reduce both iron (Fe3+) and copper (Cu2+) cations. This protein may mediate downstream responses to p53, including promoting apoptosis. Deficiency in this gene can cause anemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]

Uniprot Description

Endosomal ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells. Participates in erythroid iron homeostasis by reducing Fe3+ to Fe2+. Can also reduce of Cu2+ to Cu1+, suggesting that it participates in copper homeostasis. Uses NADP+ as acceptor. May play a role downstream of p53/TP53 to interface apoptosis and cell cycle progression. Indirectly involved in exosome secretion by facilitating the secretion of proteins such as TCTP.