Rabbit anti-Human STEAP3 Polyclonal Antibody | anti-STEAP3 antibody

STEAP3, CT (STEAP3, TSAP6, Metalloreductase STEAP3, Dudulin-2, Six-transmembrane epithelial antigen of prostate 3, Tumor suppressor-activated pathway protein 6, pHyde) (Azide free) (HRP)

Cat. Num. AAA6351943

• Gene Names: STEAP3; STMP3; TSAP6; AHMIO2; dudlin-2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: STEAP3; Polyclonal Antibody; CT (STEAP3; TSAP6; Metalloreductase STEAP3; Dudulin-2; Six-transmembrane epithelial antigen of prostate 3; Tumor suppressor-activated pathway protein 6; pHyde) (Azide free) (HRP); pHyde; anti-STEAP3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-STEAP3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: STEAP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 440-470 amino acids from the C-terminal region of human STEAP3.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(STEAP3 Antibody (C-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the STEAP3 antibody detected the STEAP3 protein (arrow).)

Related Product Information for anti-STEAP3 antibody

Endosomal ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells. Participates in erythroid iron homeostasis by reducing Fe(3+) to Fe(2+). Can also reduce of Cu(2+) to Cu(1+), suggesting that it participates in copper homeostasis. Uses NAD(+) as acceptor (By similarity). May play a role downstream of p53/TP53 to interface apoptosis and cell cycle progression. Indirectly involved in exosome secretion by facilitating the secretion of proteins such as TCTP.

Product Categories/Family for anti-STEAP3 antibody

Antibodies; Transmembrane Proteins

References

Ucisik-Akkaya, E., et al. Mol. Hum. Reprod. 16(10):770-777(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Caillot, F., et al. Histopathology 54(3):319-327(2009). Sendamarai, A.K., et al. Proc. Natl. Acad. Sci. U.S.A. 105(21):7410-7415(2008). Olsen, J.V., et al. Cell 127(3):635-648(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 56549147
• NCBI GeneID: 55240
• NCBI Accession #: NP_001008410.1; NP_878919.2
• NCBI GenBank Nucleotide #: NM_001008410.1
• UniProt Accession #: Q658P3; Q4VBR2; Q4ZG36; Q53SQ8; Q7Z389; Q86SF6; Q8NEW6; Q8TDP3; Q8TF03; Q9NVB5; A8K6E3
• Molecular Weight: 54,601 Da
• NCBI Official Full Name: metalloreductase STEAP3 isoform b
• NCBI Official Synonym Full Names: STEAP family member 3, metalloreductase
• NCBI Official Symbol: STEAP3
• NCBI Official Synonym Symbols: STMP3; TSAP6; AHMIO2; dudlin-2
• NCBI Protein Information: metalloreductase STEAP3; pHyde; hTSAP6; hpHyde; dudlin 2; dudulin 2; dudulin-2; 1010001D01Rik; tumor suppressor pHyde; six transmembrane prostate protein 3; tumor suppressor-activated pathway protein 6; six-transmembrane epithelial antigen of prostate 3
• UniProt Protein Name: Metalloreductase STEAP3
• Protein Family: Metalloreductase
• UniProt Gene Name: STEAP3
• UniProt Synonym Gene Names: TSAP6; hTSAP6; hpHyde
• UniProt Entry Name: STEA3_HUMAN

NCBI Description

This gene encodes a multipass membrane protein that functions as an iron transporter. The encoded protein can reduce both iron (Fe3+) and copper (Cu2+) cations. This protein may mediate downstream responses to p53, including promoting apoptosis. Deficiency in this gene can cause anemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]

Uniprot Description

STEAP3: a multi-pass membrane protein induced directly by p53. Plays an important role in facilitating exosome secretion. Enhances secretion in the DNA damage-induced p53-dependent exosomal secretory pathway. Localizes to vesicular-like structures at the plasma membrane and around the nucleus. Highly expressed in hematopoietic tissues where it localizes to the specialized endosome. An endosomal ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells. Participates in erythroid iron homeostasis by reducing Fe(3+) to Fe(2+). Can also reduce of Cu(2+) to Cu(1+), suggesting that it participates in copper homeostasis. Uses NAD(+) as acceptor. Contains a highly-conserved tyrosine-based motif that has been shown to target transmembrane proteins, including LAMP-1, LAMP-2, the TfR, and SLC11A1 to endosomes, lysosomes, and lysosome-related organelles. Facilitates the exosomal secretion of proteins such as TCTP. Interacts with BNIP3L, MYT1 and TPT1. Four human isoforms are produced by alternative splicing. Isoform 4 is also known as pHyde II.

Protein type: Membrane protein, integral; Oxidoreductase; Membrane protein, multi-pass; Transporter, ion channel; Transporter, iron; EC 1.16.1.-; Transporter

Chromosomal Location of Human Ortholog: 2q14.2

Cellular Component: multivesicular body; integral to membrane; plasma membrane; endosome membrane

Molecular Function: protein binding; metal ion binding; cupric reductase activity; ferric-chelate reductase activity

Biological Process: apoptosis; cellular iron ion homeostasis; protein secretion; copper ion import; transferrin transport; cell cycle; transmembrane transport

Disease: Anemia, Hypochromic Microcytic, With Iron Overload 2