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Western Blot (WB) (Western blot analysis of 1) Hela, 2)Mouse Brain Tissue, 3) Rat Brain tissue with diluted at 1:2, 000.)

Mouse Vimentin Monoclonal Antibody | anti-VIM antibody

Anti-Vimentin Mouse mAb

Reactivity
Human, Rat, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Fractional precipitation
Synonyms
Vimentin; Monoclonal Antibody; Anti-Vimentin Mouse mAb; FLJ36605 antibody; VIM antibody; VIME antibody; anti-VIM antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Rat, Mouse
Clonality
Monoclonal
Purity/Purification
Fractional precipitation
Form/Format
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Sequence Length
466
Applicable Applications for anti-VIM antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:1,000-2,000
ICC: 1:100-200
IHC: 1:200-500
Immunogen
Recombinant Protein
Preparation and Storage
Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of 1) Hela, 2)Mouse Brain Tissue, 3) Rat Brain tissue with diluted at 1:2, 000.)

Western Blot (WB) (Western blot analysis of 1) Hela, 2)Mouse Brain Tissue, 3) Rat Brain tissue with diluted at 1:2, 000.)

Immunofluorescence (IF)

(IF analysis of Hela with (Left) and DAPI (Right) diluted at 1:100.)

Immunofluorescence (IF) (IF analysis of Hela with (Left) and DAPI (Right) diluted at 1:100.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast caricnoma using Vimentin uff08uff09Mouse mAb diluted at 1:500.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human breast caricnoma using Vimentin uff08uff09Mouse mAb diluted at 1:500.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50-57
NCBI Official Full Name
vimentin
NCBI Official Synonym Full Names
vimentin
NCBI Official Symbol
VIM
NCBI Protein Information
vimentin
UniProt Protein Name
Vimentin
Protein Family
UniProt Gene Name
VIM

NCBI Description

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]

Uniprot Description

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

Research Articles on VIM

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Product Notes

The VIM vim (Catalog #AAA477278) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Vimentin Mouse mAb reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Vimentin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:1,000-2,000 ICC: 1:100-200 IHC: 1:200-500. Researchers should empirically determine the suitability of the VIM vim for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Vimentin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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