Rabbit anti-Human, Mouse STAT6 Polyclonal Antibody | anti-STAT6 antibody

Rabbit anti STAT6 (pT645) Polyclonal Antibody

Cat. Num. AAA460081

• Gene Names: STAT6; STAT6B; STAT6C; D12S1644; IL-4-STAT
• Reactivity: Human, Mouse
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by site-modified Epitope Affinity Purification.
• Synonyms: STAT6; Polyclonal Antibody; Rabbit anti STAT6 (pT645) Polyclonal Antibody; Signal transducer and activator of transcription 6 (STAT6); anti-STAT6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody recognizes STAT6 (pT645) with a phosphorylated sites of THR645. It does not cross-react with non-phosphospecific peptide.
• Purity/Purification: Purified by site-modified Epitope Affinity Purification.
• Form/Format: Supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
• Sequence Length: 737

• Applicable Applications for anti-STAT6 antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: ELISA: 0.01-0.1 ug/ml; Flow Cytometry: 5-10 ug/ml; Immunohistochemistry: 2-10 ug/ml; Immunoprecipitation: 2-5 ug/ml; Western Blot: 0.1-1 ug/ml
• Preparation: A synthetic peptide surrounding to the epitope with phosphorylation sites at Thr645 of STAT6 protein from human, mouse and rat origins.
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C. The antibodies can be stored at 2 degree C - 8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Related Product Information for anti-STAT6 antibody

STAT proteins (Signal transducer and activator of transcription) belong to a family of cytoplasmic transcription factors that can be phosphorylated by a ligand binding to its cell surface receptor. Activation by phosphorylation of STAT6 at tyrosine 641 upon the IL-4 (possibly IL-13) ligand binding to its receptor induces dimerization, nuclear translocation and DNA binding. STAT6 is widely expressed in human tissues. It exhibits elevated expression in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung, and placenta. STAT6 mediates the IL-4 signal and is essential for the proper development of adaptive immunity

References

Zhiguang Li et al (2008). Endogenous Interleukin-4 Promotes Tumor Development by Increasing Tumor Cell Resistance to Apoptosis. Cancer Research 68, 8687-94.

NCBI and Uniprot Product Information

• NCBI GI #: 296010870
• NCBI GeneID: 6778
• NCBI Accession #: NP_001171552.1
• NCBI GenBank Nucleotide #: NM_001178081.1
• Molecular Weight: 81,748 Da
• NCBI Official Full Name: signal transducer and activator of transcription 6 isoform 2
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 6
• NCBI Official Symbol: STAT6
• NCBI Official Synonym Symbols: STAT6B; STAT6C; D12S1644; IL-4-STAT
• NCBI Protein Information: signal transducer and activator of transcription 6
• UniProt Protein Name: Signal transducer and activator of transcription 6
• Protein Family: Signal transducer and transcription activator
• UniProt Gene Name: STAT6

NCBI Description

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Uniprot Description

STAT6: transcription factor of the STAT family. Plays a central role in IL4-mediated biological responses. Induces the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. May function in the differentiation of T helper 2 cells and class switch of immunoglobulins. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: cytosol; nuclear membrane; nucleoplasm

Molecular Function: identical protein binding; protein binding; protein phosphatase binding; transcription factor activity

Biological Process: positive regulation of interferon type I production; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; signal transduction