Mouse anti-Human E-cadherin Monoclonal Antibody | anti-CDH1 antibody

E-cadherin Antibody

Cat. Num. AAA346012

• Gene Names: CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324
• Reactivity: Human
• Applications: Immunohistochemistry
• Synonyms: E-cadherin; Monoclonal Antibody; E-cadherin Antibody; anti-CDH1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: IHC564
• Form/Format: Liquid
• Sequence Length: 827

• Applicable Applications for anti-CDH1 antibody: Immunohistochemistry (IHC) Paraffin
• Application Notes: Dilution Range: 1:100 - 1:200
• Buffer: Tris Buffer, ph7.4, 1% BSA, <0.1% NaN3
• IHC Positive Control: Breast
• IHC Suggested Protocol: 1. Tissues are fixed with 10% neutral buffered formalin, cut to ~4um, and placed on a positively charged glass slide.; 2. The prepared slide is baked for at least 30 minutes and at most 24 hours at 55-65 degree C.; 3. Pretreatment - Perform heat-induced epitope retrieval (HIER) at pH 6 for 10 to 30 minutes.; 4. Block – Block with peroxidase solution for 5 minutes at RT.; 5. Primary Ab – Dilute antibodies 1:100 – 1:200 and incubate for 10 - 30 minutes at RT.; 6. Secondary Ab - Incubate for 20 - 30 minutes at RT.; 7. Substrate - Incubate DAB or Fast Red for 5 - 10 minutes at RT.; 8. Counterstain - Counterstain with hematoxylin for 30 seconds to 5 minutes, rinse with distilled water and blueing solution for 30 seconds.; 9. Dehydrate and apply coverslip.; (Each researcher should optimize the protocol for their experimental model system.)
• Preparation and Storage: Store at 2-8 degree C. Do not freeze.

Immunohistochemistry (IHC)

(IHC (paraffin) of human breast using E-cadherin Antibody)

Related Product Information for anti-CDH1 antibody

E-cadherin is an important intercellular adhesion protein used to form junctions between cells to bind them together. Present in epithelial cells, anti-E-cadherin antibodies stain the glandular epithelium, as well as lung, gastrointestinal, and ovarian adenocarcinomas, and some thyroid cancers.

NCBI and Uniprot Product Information

• NCBI GI #: 1617084
• NCBI GeneID: 999
• UniProt Accession #: P12830; Q13799; Q14216; Q15855; Q16194; Q4PJ14; Q9UII8; A8K1U7
• Molecular Weight: 90,942 Da
• NCBI Official Full Name: E-cadherin, partial
• NCBI Official Synonym Full Names: cadherin 1
• NCBI Official Symbol: CDH1
• NCBI Official Synonym Symbols: UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324
• NCBI Protein Information: cadherin-1
• UniProt Protein Name: Cadherin-1
• Protein Family: Cadherin
• UniProt Gene Name: CDH1
• UniProt Synonym Gene Names: CDHE; UVO; E-cadherin

NCBI Description

This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015]

Uniprot Description

Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.