Western Blot (WB)
(HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human STAT4 (RC206892) with C-terminal MYC tag probed (0.5ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.)
Goat STAT4 Polyclonal Antibody | anti-STAT4 antibody
Goat anti-STAT4 (aa 613-627) Antibody
Western Blot: In transfected HEK293 transiently expressing full-length Human STAT4 (MYC and DYKDDDDK tagged), a band of approx.85kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.2-0.5ug/ml.
Western Blot (WB)
(HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human STAT4 (RC206892) with C-terminal MYC tag probed (0.5ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
(HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human STAT4 (RC206892) with C-terminal MYC tag probed (0.5ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. Mutations in this gene may be associated with systemic lupus erythematosus and rheumatoid arthritis. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011]
Uniprot Description
STAT4: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells.
Protein type: Transcription factor; DNA-binding
Chromosomal Location of Human Ortholog: 2q32.2-q32.3
Cellular Component: cytoplasm; nucleoplasm
Molecular Function: protein binding; transcription factor activity
Biological Process: JAK-STAT cascade; regulation of transcription, DNA-dependent; transcription, DNA-dependent
Disease: Systemic Lupus Erythematosus, Susceptibility To, 11
Research Articles on STAT4
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Product Notes
The STAT4 stat4 (Catalog #AAA422311) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-STAT4 (aa 613-627) Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Dog, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's STAT4 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB-Trf). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: In transfected HEK293 transiently expressing full-length Human STAT4 (MYC and DYKDDDDK tagged), a band of approx.85kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.2-0.5ug/ml. Researchers should empirically determine the suitability of the STAT4 stat4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DHSESGEVRF HSVEP. It is sometimes possible for the material contained within the vial of "STAT4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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