Rat Protease Activated Receptor 2 ELISA Kit | PAR2 elisa kit

Rat Protease Activated Receptor 2 ELISA Kit

Cat. Num. AAA720849

• Gene Names: F2RL1; PAR2; GPR11
• Reactivity: Rat
• Synonyms: Protease Activated Receptor 2; Rat Protease Activated Receptor 2 ELISA Kit; PAR2 elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive or Sandwich
• Detection Range: 25-500 uIU/L
• Sensitivity: 1.0 uIU/L
• Intended Uses: This PAR2ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat PAR2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PAR2 elisa kit

Principle of the Assay: PAR2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PAR2. Standards or samples are then added to the microtiter plate wells and PAR2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PAR2 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for PAR2 are added to each well to "sandwich" the PAR2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PAR2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PAR2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for PAR2 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 34577052
• NCBI GeneID: 2150
• NCBI Accession #: NP_005233.3
• NCBI GenBank Nucleotide #: NM_005242.4
• Molecular Weight: 44,126 Da
• NCBI Official Full Name: proteinase-activated receptor 2
• NCBI Official Synonym Full Names: coagulation factor II (thrombin) receptor-like 1
• NCBI Official Symbol: F2RL1
• NCBI Official Synonym Symbols: PAR2; GPR11
• NCBI Protein Information: proteinase-activated receptor 2; thrombin receptor-like 1; G-protein coupled receptor 11; protease-activated receptor 2; coagulation factor II receptor-like 1
• UniProt Protein Name: Proteinase-activated receptor 2
• Protein Family: Transcription factor
• UniProt Gene Name: F2RL1
• UniProt Synonym Gene Names: GPR11; PAR2; PAR-2
• UniProt Entry Name: PAR2_HUMAN

NCBI Description

Coagulation factor II (thrombin) receptor-like 1 (F2RL1) is a member of the large family of 7-transmembrane-region receptors that couple to guanosine-nucleotide-binding proteins. F2RL1 is also a member of the protease-activated receptor family. It is activated by trypsin, but not by thrombin. It is activated by proteolytic cleavage of its extracellular amino terminus. The new amino terminus functions as a tethered ligand and activates the receptor. The F2RL1 gene contains two exons and is widely expressed in human tissues. The predicted protein sequence is 83% identical to the mouse receptor sequence. [provided by RefSeq, Jul 2008]

Uniprot Description

F2RL1: a G-protein coupled receptor for trypsin and trypsin-like enzymes. Acts as a sensor for proteolytic enzymes generated during infection. Modulates pro-inflammatory responses, and innate and adaptive immunity. It is activated by proteolytic cleavage of its extracellular amino terminus. The new amino terminus functions as a tethered ligand and activates the receptor. Activates several signaling molecules including phospholipase C (PLC), mitogen-activated protein kinase (MAPK), IKK/NFkB and Rho. Elevates intracellular calcium. Can also be transactivated by cleaved PAR1. Can signal synergistically with TLR4 and probably TLR2 in inflammatory responses and modulates TLR3 signaling. Has a protective role in establishing the endothelial barrier; the activity involves coagulation factor X. Proposed to have a bronchoprotective role in airway epithelium, but also shown to compromise the airway epithelial barrier by interrupting E-cadherin adhesion. Involved in the regulation of vascular tone; activation results in hypotension presumably mediated by vasodilation. Associates with a subset of G proteins alpha subunits such as G alpha-q, G alpha-11, G alpha-14, G alpha- 12 and G alpha-13, but probably not with G(o) alpha, G(i) subunit alpha-1 and G(i) subunit alpha-2. However, may signal through G(i) subunit alpha. Believed to be a class B receptor that internalizes as a complex with arrestin and traffic with it to endosomal vesicles, presumably as desensitized receptor, for extended periods of time. Mediates inhibition of TNF-alpha stimulated JNK phosphorylation via coupling to G alpha-q/11; the function involves dissociation of RIPK1 and TRADD from TNFR1. Involved in cellular migration. Involved in cytoskeletal rearrangement and chemotaxis through beta-arrestin-promoted scaffolds; the function is independent of G alpha-q/11 and involves promotion of cofilin dephosphoryltaion and actin filament severing. Induces redistribution of COPS5 from the plasma membrane to the cytosol and activation of the JNK cascade is mediated by COPS5. Involved in the recruitment of leukocytes to the sites of inflammation and is the major PAR receptor capable of modulating eosinophil function such as proinflammatory cytokine secretion, superoxide production and degranulation. During inflammation promotes dendritic cell maturation, trafficking to the lymph nodes and subsequent T-cell activation. Involved in antimicrobial response of innate immune cells; activation enhances phagocytosis of Gram- positive and killing of Gram-negative bacteria. Acts synergistically with interferon-gamma in enhancing antiviral responses. Implicated in a number of acute and chronic inflammatory diseases such as of the joints, lungs, brain, gastrointestinal tract, periodontium, skin, and vascular systems, and in autoimmune disorders. Widely expressed in tissues with especially high levels in pancreas, liver, kidney, small intestine, and colon. Moderate expression is detected in many organs, but none in brain or skeletal muscle. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral; GPCR, family 1

Chromosomal Location of Human Ortholog: 5q13

Cellular Component: Golgi apparatus; integral to plasma membrane; early endosome; plasma membrane; pseudopodium

Molecular Function: G-protein coupled receptor activity; protein binding; G-protein alpha-subunit binding; G-protein beta-subunit binding; receptor activity; thrombin receptor activity; receptor binding

Biological Process: negative regulation of JNK cascade; positive regulation of positive chemotaxis; positive regulation of cytokine secretion during immune response; positive regulation of JNK cascade; positive regulation of leukocyte chemotaxis; positive regulation of vasodilation; regulation of JNK cascade; negative regulation of toll-like receptor 3 signaling pathway; T cell activation during immune response; elevation of cytosolic calcium ion concentration; positive regulation of glomerular filtration; positive regulation of superoxide release; interleukin-1 beta secretion; regulation of I-kappaB kinase/NF-kappaB cascade; inflammatory response; defense response to virus; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of toll-like receptor 3 signaling pathway; neutrophil activation; positive regulation of actin filament depolymerization; positive regulation of eosinophil degranulation; positive regulation of chemotaxis; positive regulation of toll-like receptor 4 signaling pathway; positive regulation of Rho protein signal transduction; positive regulation of toll-like receptor 2 signaling pathway; positive regulation of phosphoinositide 3-kinase cascade; G-protein coupled receptor protein signaling pathway; positive regulation of pseudopodium formation; regulation of blood coagulation; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of phagocytosis, engulfment; blood coagulation; leukocyte migration; positive regulation of cell migration