Rabbit anti-Human STAT1 Polyclonal Antibody | anti-Stat1 antibody

STAT1, Phosphorylated (Ser727) (Signal Transducer and Activator of Transcription 1-alpha/beta, Transcription Factor ISGF-3 Components p91/p84)

Cat. Num. AAA643778

• Gene Names: Stat1; AA408197; 2010005J02Rik
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: STAT1; Polyclonal Antibody; Phosphorylated (Ser727) (Signal Transducer and Activator of Transcription 1-alpha/beta; Transcription Factor ISGF-3 Components p91/p84); Anti -STAT1; anti-Stat1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human STAT1 when phosphorylated at Ser727. Species sequence homology: mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-Stat1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: ELISA: 1:10,000; Western Blot: 1:500-1:1000; Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
• Immunogen: Synthetic phosphopeptide corresponding to human STAT1 around the phosphorylation site of Ser727.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Intestine, Ganglion Cells: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry (IHC)

(Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(Western blot analysis of extracts from 293 cells, 3T3 cells treated with UV (15mins) and Jurkat cells treated with ETO (25uM, 24hours), using STAT1 (Phospho-Ser727) Antibody. The lane on the right is treated with the synthesized peptide.)

Related Product Information for anti-Stat1 antibody

STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a, Stat5b, and Stat6), Stat1, Stat3, Stat5a, and Stat5b have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-a, IFN-b, IFN-g and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). STAT1 has two forms, the 91kD STAT1a and the 84kD STAT1b which are encoded by the same gene with splicing variant.

Product Categories/Family for anti-Stat1 antibody

Antibodies; Abs to Transcription Factors; STAT

NCBI and Uniprot Product Information

• NCBI GI #: 458710
• NCBI GeneID: 20846
• UniProt Accession #: P42225
• Molecular Weight: 87,197 Da
• NCBI Official Full Name: Stat1
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 1
• NCBI Official Symbol: Stat1
• NCBI Official Synonym Symbols: AA408197; 2010005J02Rik
• NCBI Protein Information: signal transducer and activator of transcription 1
• UniProt Protein Name: Signal transducer and activator of transcription 1
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: Stat1
• UniProt Entry Name: STAT1_MOUSE

Uniprot Description

STAT1: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of certain receptor tyrosine kinases, GPCRs, and receptors for various interleukins and interferons. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.

Protein type: Transcription factor; DNA-binding

Cellular Component: nucleoplasm; axon; nuclear chromatin; cytoplasm; dendrite; nucleolus; nucleus

Molecular Function: identical protein binding; signal transducer activity; protein binding; enzyme binding; protein homodimerization activity; DNA binding; protein phosphatase 2A binding; sequence-specific DNA binding; double-stranded DNA binding; tumor necrosis factor receptor binding; transcription factor activity; CCR5 chemokine receptor binding

Biological Process: transcription from RNA polymerase II promoter; response to peptide hormone stimulus; response to cAMP; blood circulation; positive regulation of transcription, DNA-dependent; positive regulation of smooth muscle cell proliferation; response to lipopolysaccharide; signal transduction; response to exogenous dsRNA; tumor necrosis factor-mediated signaling pathway; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; negative regulation of viral protein levels in host cell; lipopolysaccharide-mediated signaling pathway; response to nutrient; caspase activation; response to drug; transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; negative regulation of I-kappaB kinase/NF-kappaB cascade; JAK-STAT cascade; negative regulation of angiogenesis; cellular response to insulin stimulus; response to bacterium; response to mechanical stimulus; response to cytokine stimulus; negative regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter