Rabbit STAT 5a,b Polyclonal Antibody | anti-STAT5A antibody

STAT 5a,b (Signal Transducer and Activator of Transcription 5a,b)

Cat. Num. AAA621216

• Gene Names: STAT5A; MGF; STAT5
• Reactivity: Human, Mouse, Rat, Sheep
• Purity: Serum; Whole rabbit antiserum
• Synonyms: STAT 5a; b; Polyclonal Antibody; b (Signal Transducer and Activator of Transcription 5a; b); Anti -STAT 5a; anti-STAT5A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Sheep
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human STAT5 A & B; crossreactivity unknown, although the immunogenic sequence is identical in mouse, rat and sheep.
• Purity/Purification: Serum; Whole rabbit antiserum
• Form/Format: Frozen serum

• Application Notes: Antibody Supershift: An Independent laboratory showed that Anti-STAT5 (A&B) supershifted IL-2 induced complexes in both fresh and preactivated peripheral blood lymphocytes. This antibody also supershifted the complexes induced by IL-2, IL-7 and IL-15, but not those induced by IL-4 and IL-13 in fresh peripheral blood lymphocytes. The specificity of the reaction was confirmed by competition with the immunogenic peptide (Lin, et al., 1995) Experimental conditions must be optimized to achieve the best supershift banding pattern (see Lin, et al., 1993).; Immunohistochemistry: Not tested. ; Immunoprecipitation: Not recommended. Immunocytochemistry: Not tested.
• Immunogen: KLH conjugated synthetic peptide (C-QELQKKAEHQVGEDG), corresponding to amino acids 66-80 of Human STAT5 A&B, with the N-terminal cysteine having been added.
• Preparation and Storage: -20 degree C

Related Product Information for anti-STAT5A antibody

STAT (signal transducers and activators of transcription) proteins can be selectively phosphorylated on tyrosine residues by Jak family kinases and some receptors with tyrosine kinase activity. The STAT proteins are highly conserved in their amino termini but have divergent carboxy termini, which are thought to be essential for their selective activity. STAT5 can be activated by a variety of agents including prolactin, IL-2, IL-3, IL-7, IL-15, growth hormone and erythropoietin. In peripheral blood lymphocytes (PBL), IL-2 rapidly activates STAT5 while phytohemagglutinin activates both STAT3 and STAT5. Evidence indicates that both STAT5A and STAT5B are activated in PBL in vivo. Data show that either Tyr-392 or Tyr-510 of the IL-2Rb is required for IL-2 dependent activation of STAT5. JAK1 and JAK3 may activate each STAT5 isoform by phosphorylation of a single tyrosine residue.

Product Categories/Family for anti-STAT5A antibody

Antibodies; Abs to Transcription Factors; STAT

NCBI and Uniprot Product Information

• NCBI GI #: 21618342
• NCBI GeneID: 6776
• NCBI Accession #: NP_003143.2
• NCBI GenBank Nucleotide #: NM_003152.3
• UniProt Accession #: P42229; Q1KLZ6; Q59GY7; Q8WWS9
• Molecular Weight: 90,647 Da
• NCBI Official Full Name: signal transducer and activator of transcription 5A
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 5A
• NCBI Official Symbol: STAT5A
• NCBI Official Synonym Symbols: MGF; STAT5
• NCBI Protein Information: signal transducer and activator of transcription 5A; OTTHUMP00000198376
• UniProt Protein Name: Signal transducer and activator of transcription 5A
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: STAT5A
• UniProt Synonym Gene Names: STAT5
• UniProt Entry Name: STA5A_HUMAN

NCBI Description

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. [provided by RefSeq]

Uniprot Description

STAT5A: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 fusion protein is essential for the tumorigenesis. Induces the expression of BCL2L1/BCL-X(L) in the mouse, suggesting an antiapoptotic function of this protein. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.

Protein type: DNA-binding; Motility/polarity/chemotaxis; Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: nucleoplasm; cytosol

Molecular Function: protein binding; signal transducer activity; transcription factor activity

Biological Process: lactation; succinate metabolic process; oxaloacetate metabolic process; peptidyl-tyrosine phosphorylation; positive regulation of interleukin-2 biosynthetic process; T cell differentiation in the thymus; female pregnancy; positive regulation of multicellular organism growth; positive regulation of activated T cell proliferation; positive regulation of mitotic cell cycle; fatty acid metabolic process; 2-oxoglutarate metabolic process; positive regulation of natural killer cell differentiation; sequestering of lipid; allantoin metabolic process; natural killer cell differentiation; negative regulation of mast cell apoptosis; luteinization; development of secondary male sexual characteristics; regulation of steroid metabolic process; creatinine metabolic process; T cell homeostasis; positive regulation of gamma-delta T cell differentiation; Peyer's patch development; isoleucine metabolic process; negative regulation of erythrocyte differentiation; transcription, DNA-dependent; citrate metabolic process; valine metabolic process; regulation of multicellular organism growth; development of secondary female sexual characteristics; JAK-STAT cascade; positive regulation of natural killer cell mediated cytotoxicity; creatine metabolic process; regulation of transcription from RNA polymerase II promoter; regulation of epithelial cell differentiation; positive regulation of B cell differentiation; positive regulation of transcription from RNA polymerase II promoter; taurine metabolic process; positive regulation of inflammatory response