Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein FAM3B (FAM3B) Standard Curve.)
Human Protein FAM3B (FAM3B) ELISA Kit | FAM3B elisa kit
Human Protein FAM3B (FAM3B) ELISA Kit
Gene Names
FAM3B; 2-21; ORF9; PANDER; PRED44; C21orf11; C21orf76
Reactivity
Human
Synonyms
Protein FAM3B (FAM3B); Human Protein FAM3B (FAM3B) ELISA Kit; FAM3B; 2-21; C21orf11; C21orf76; ORF9; PANDER; PRED44; D21M16SJHU19e; cytokine-like protein 2-21; pancreatic derived factor; FAM3B elisa kit
Reactivity
Human
Specificity
Human Protein FAM3B (FAM3B) ELISA Kit has high sensitivity and excellent specificity for detection of Human FAM3B. No significant cross-reactivity or interference between Human FAM3B and analogues was observed.
Sequence Length
187
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein FAM3B (FAM3B) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein FAM3B (FAM3B) Standard Curve.)
Related Product Information for FAM3B elisa kit
Description: This Human Protein FAM3B (FAM3B) ELISA Kit employs a two-site sandwich ELISA to quantitate FAM3B in samples. An antibody specific for FAM3B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FAM3B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FAM3B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FAM3B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The authors predicted that FAM3B forms a 4-helix bundle, and 3-dimensional modeling indicated that 4 cysteines could form 2 disulfide bonds linking helices 1 and 4 and helices 2 and 3. The human and mouse FAM3B proteins share 78% amino acid identity. Northern blot analysis detected FAM3B expression at a high level in pancreas and at lower levels in small intestine and prostate. Immunohistochemical analysis of mouse tissues detected prominent Fam3b staining in the endocrine pancreas, specifically in the islets of Langerhans. Staining was also found in round spermatids within the seminiferous tubules, in the Purkinje cell layer of the cerebellum, and in nerve cell bodies of numerous brainstem nuclei. Transfected Chinese hamster ovary cells secreted Fam3b to the culture medium.
Background: The authors predicted that FAM3B forms a 4-helix bundle, and 3-dimensional modeling indicated that 4 cysteines could form 2 disulfide bonds linking helices 1 and 4 and helices 2 and 3. The human and mouse FAM3B proteins share 78% amino acid identity. Northern blot analysis detected FAM3B expression at a high level in pancreas and at lower levels in small intestine and prostate. Immunohistochemical analysis of mouse tissues detected prominent Fam3b staining in the endocrine pancreas, specifically in the islets of Langerhans. Staining was also found in round spermatids within the seminiferous tubules, in the Purkinje cell layer of the cerebellum, and in nerve cell bodies of numerous brainstem nuclei. Transfected Chinese hamster ovary cells secreted Fam3b to the culture medium.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein FAM3B isoform b
NCBI Official Synonym Full Names
family with sequence similarity 3 member B
NCBI Official Symbol
FAM3B
NCBI Official Synonym Symbols
2-21; ORF9; PANDER; PRED44; C21orf11; C21orf76
NCBI Protein Information
protein FAM3B
UniProt Protein Name
Protein FAM3B
Protein Family
UniProt Gene Name
FAM3B
UniProt Synonym Gene Names
C21orf11; C21orf76; PANDER
UniProt Entry Name
FAM3B_HUMAN
Uniprot Description
FAM3B: Induces apoptosis of alpha and beta cells in a dose- and time-dependent manner. Present in insulin secretory granules and likely cosecreted with insulin. Belongs to the FAM3 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Nuclear envelope; Secreted, signal peptide; Apoptosis; Cytokine
Chromosomal Location of Human Ortholog: 21q22.3
Cellular Component: extracellular space; extracellular region
Molecular Function: cytokine activity
Biological Process: insulin secretion; apoptosis
Research Articles on FAM3B
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Product Notes
The Human FAM3B fam3b (Catalog #AAA9716055) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716055 ELISA Kit recognizes Human FAM3B. It is sometimes possible for the material contained within the vial of "Protein FAM3B (FAM3B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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