Rabbit anti-Human STAM Polyclonal Antibody | anti-STAM antibody

STAM, CT (STAM-1, Signal Transducing Adapter Molecule 1, STAM1) (MaxLight 650)

Cat. Num. AAA6500799

• Gene Names: STAM; STAM1; STAM-1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: STAM; Polyclonal Antibody; CT (STAM-1; Signal Transducing Adapter Molecule 1; STAM1) (MaxLight 650); anti-STAM antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human STAM.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-STAM antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 326-356 in the C-terminal region of human STAM. Species sequence homology: mouse
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in Hela cell line lysates (35ug/lane) usingMBS642958. STAM (arrow) was detected usingMBS642958.)

Western Blot (WB)

(Western Blot analysis of STAM (arrow) in 293 cell lysates (2ug/lane) using MBS642958 either nontransfected (Lane 1) or transiently transfected (Lane 2) with the STAM gene.)

Product Categories/Family for anti-STAM antibody

Antibodies; Adapter Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 71153545
• NCBI GeneID: 8027
• UniProt Accession #: Q92783; Q8N6D9; B0YJ99; D3DRU5
• Molecular Weight: 540
• NCBI Official Full Name: Signal transducing adapter molecule 1
• NCBI Official Synonym Full Names: signal transducing adaptor molecule (SH3 domain and ITAM motif) 1
• NCBI Official Symbol: STAM
• NCBI Official Synonym Symbols: STAM1; STAM-1
• NCBI Protein Information: signal transducing adapter molecule 1; HSE1 homolog
• UniProt Protein Name: Signal transducing adapter molecule 1
• Protein Family: STAM-binding protein
• UniProt Gene Name: STAM
• UniProt Synonym Gene Names: STAM1; STAM-1
• UniProt Entry Name: STAM1_HUMAN

NCBI Description

This gene encodes a member of the signal-transducing adaptor molecule family. These proteins mediate downstream signaling of cytokine receptors and also play a role in ER to Golgi trafficking by interacting with the coat protein II complex. The encoded protein also associates with hepatocyte growth factor-regulated substrate to form the endosomal sorting complex required for transport-0 (ESCRT-0), which sorts ubiquitinated membrane proteins to the ESCRT-1 complex for lysosomal degradation. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

STAM1: Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. Belongs to the STAM family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 10p14-p13

Cellular Component: early endosome membrane; cytosol

Molecular Function: protein binding; SH3/SH2 adaptor activity

Biological Process: negative regulation of epidermal growth factor receptor signaling pathway; epidermal growth factor receptor signaling pathway; intracellular protein transport; positive regulation of signal transduction; signal transduction; endosome transport