Rabbit anti-Human, Mouse ST14 Polyclonal Antibody | anti-ST14 antibody

ST14, CT (ST14, PRSS14, SNC19, TADG15, Suppressor of tumorigenicity 14 protein, Matriptase, Membrane-type serine protease 1, Prostamin, Serine protease 14, Serine protease TADG-15, Tumor-associated differentially-expressed gene 15 protein) (MaxLight 490)

Cat. Num. AAA6351494

• Gene Names: ST14; HAI; MTSP1; SNC19; ARCI11; MT-SP1; PRSS14; TADG15; TMPRSS14
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: ST14; Polyclonal Antibody; CT (ST14; PRSS14; SNC19; TADG15; Suppressor of tumorigenicity 14 protein; Matriptase; Membrane-type serine protease 1; Prostamin; Serine protease 14; Serine protease TADG-15; Tumor-associated differentially-expressed gene 15 protein) (MaxLight 490); Tumor-associated differentially-expressed gene 15 protein; anti-ST14 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ST14. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-ST14 antibody: Western Blot (WB), Immunohistochemistry (IHC), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 826-855 in the C-terminal region of human ST14, UniProt Accession #Q9Y5Y6.
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot of PC-3 whole cell lysate Lysates/proteins (20ug/lane) using MBS6000692 at 1:1000 dilution. Secondary IgG (H&L) goat anti-rabbit, Peroxidase conjugated at 1:10000 dilution. Predicted band size : 95kD)

Western Blot (WB)

(Western Blot analysis in mouse liver tissue lysates (35ug/lane) usingMBS6000692. This demonstrates that MBS6000692 detected the ST14 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma using MBS6000692 followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MBS6000692 for immunohistochemistry.)

Related Product Information for anti-ST14 antibody

ST14 is an epithelial-derived, integral membrane serine protease. This protease forms a complex with the Kunitz-type serine protease inhibitor, HAI-1, and is found to be activated by sphingosine 1-phosphate. This protease has been shown to cleave and activate hepatocyte growth factor/scattering factor, and urokinase plasminogen activator, which suggest the function of this protease as an epithelial membrane activator for other proteases and latent growth factors. The expression of this protease has been associated with breast, colon, prostate, and ovarian tumors, which implicates its role in cancer invasion, and metastasis.

Product Categories/Family for anti-ST14 antibody

Antibodies; Enzymes; Protease (Proteinase; Peptidase)

References

1. Santin, A.D., et al., Cancer 98(9):1898-1904 (2003). 2. Oberst, M.D., et al., J. Biol. Chem. 278(29):26773-26779 (2003). 3. Ihara, S., et al., J. Biol. Chem. 277(19):16960-16967 (2002). 4. Benaud, C.M., et al., Clin. Exp. Metastasis 19(7):639-649 (2002). 5. Benaud, C., et al., J. Biol. Chem. 277(12):10539-10546 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 11415040
• NCBI GeneID: 6768
• NCBI Accession #: NP_068813
• NCBI GenBank Nucleotide #: NM_021978
• UniProt Accession #: Q9Y5Y6; Q9BS01; Q9H3S0; Q9HB36; Q9HCA3
• Molecular Weight: 94,770 Da
• NCBI Official Full Name: suppressor of tumorigenicity 14 protein
• NCBI Official Synonym Full Names: suppression of tumorigenicity 14 (colon carcinoma)
• NCBI Official Symbol: ST14
• NCBI Official Synonym Symbols: HAI; MTSP1; SNC19; ARCI11; MT-SP1; PRSS14; TADG15; TMPRSS14
• NCBI Protein Information: suppressor of tumorigenicity 14 protein; membrane-type serine protease 1; prostamin; serine protease 14; serine protease TADG-15; suppression of tumorigenicity 14 (colon carcinoma, matriptase, epithin); tumor associated differentially expressed gene 15 pr
• UniProt Protein Name: Suppressor of tumorigenicity 14 protein
• Protein Family: Suppressor of tumorigenicity 14 protein
• UniProt Gene Name: ST14
• UniProt Synonym Gene Names: PRSS14; SNC19; TADG15; MT-SP1
• UniProt Entry Name: ST14_HUMAN

Uniprot Description

ST14: Degrades extracellular matrix. Proposed to play a role in breast cancer invasion and metastasis. Exhibits trypsin-like activity as defined by cleavage of synthetic substrates with Arg or Lys as the P1 site. Defects in ST14 are a cause of ichthyosis autosomal recessive with hypotrichosis (ARIH). ARIH is a skin disorder characterized by congenital ichthyosis associated with the presence of less than the normal amount of hair. Belongs to the peptidase S1 family.

Protein type: EC 3.4.21.109; Membrane protein, integral; Protease

Chromosomal Location of Human Ortholog: 11q24-q25

Cellular Component: extrinsic to plasma membrane; extracellular space; integral to plasma membrane; basolateral plasma membrane; plasma membrane

Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: keratinocyte differentiation; proteolysis

Disease: Ichthyosis, Congenital, Autosomal Recessive 11