Human Beta-1,3-galactosyltransferase 1 ELISA Kit | B3GALT1 elisa kit

Human Beta-1,3-galactosyltransferase 1 ELISA Kit

Cat. Num. AAA761060

• Gene Names: B3galt1; beta3Gal-T1
• Reactivity: Human
• Synonyms: Beta-1; 3-galactosyltransferase 1; Human Beta-1; 3-galactosyltransferase 1 ELISA Kit; B3GALT1/Beta-1; 3-galactosyltransferase 1/Beta-1; 3-GalTase 1/Beta3Gal-T1/Beta3GalT1/UDP-galactose:beta-N-acetyl-glucosamine-beta-1; B3GALT1 elisa kit

• Reactivity: Human
• Sequence Length: 326

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Competitive ELISA, Coated with Antigen
• Detection Range: 78.125-5000pg/ml
• Sensitivity: 46.9pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for B3GALT1 elisa kit

Principle of the Assay||This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B3GALT1. During the reaction,B3GALT1 in the sample or standard competes with a fixed amount of biotin-labeled B3GALT1 for sites on a pre-coated Monoclonal antibody specific to B3GALT1. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of B3GALT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 66392577
• NCBI GeneID: 26877
• NCBI Accession #: NP_064679.2
• NCBI GenBank Nucleotide #: NM_020283.4
• Molecular Weight: 37,993 Da
• NCBI Official Full Name: beta-1,3-galactosyltransferase 1
• NCBI Official Synonym Full Names: UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 1
• NCBI Official Symbol: B3galt1
• NCBI Official Synonym Symbols: beta3Gal-T1
• NCBI Protein Information: beta-1,3-galactosyltransferase 1
• UniProt Protein Name: Beta-1,3-galactosyltransferase 1
• Protein Family: Beta-1,3-galactosyltransferase
• UniProt Gene Name: B3galt1
• UniProt Synonym Gene Names: Beta-1,3-GalTase 1; Beta3Gal-T1; Beta3GalT1
• UniProt Entry Name: B3GT1_MOUSE

Uniprot Description

B3GALT1: Beta-1,3-galactosyltransferase that transfers galactose from UDP-galactose to substrates with a terminal beta-N- acetylglucosamine (beta-GlcNAc) residue. Involved in the biosynthesis of the carbohydrate moieties of glycolipids and glycoproteins. Inactive towards substrates with terminal alpha-N- acetylglucosamine (alpha-GlcNAc) or alpha-N-acetylgalactosamine (alpha-GalNAc) residues. Belongs to the glycosyltransferase 31 family.

Protein type: Membrane protein, integral; Transferase; EC 2.4.1.-; Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series

Cellular Component: Golgi apparatus; Golgi membrane; integral to membrane; membrane

Molecular Function: 1,2-dihydroxy-phenanthrene glycosyltransferase activity; 1-phenanthrol glycosyltransferase activity; 9-phenanthrol glycosyltransferase activity; 9-phenanthrol UDP-glucuronosyltransferase activity; acetylgalactosaminyltransferase activity; acetylglucosaminyltransferase activity; alpha(1,3)-fucosyltransferase activity; alpha(1,6)-fucosyltransferase activity; alpha-1,2-galactosyltransferase activity; alpha-1,2-mannosyltransferase activity; alpha-1,3-galactosyltransferase activity; alpha-1,3-mannosyltransferase activity; alpha-1,6-mannosyltransferase activity; beta-1,4-mannosyltransferase activity; cellulose synthase activity; dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase activity; dolichyl pyrophosphate Man7GlcNAc2 alpha-1,3-glucosyltransferase activity; dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity; dolichyl-phosphate-glucose-glycolipid alpha-glucosyltransferase activity; fucosyltransferase activity; galactosyltransferase activity; glucosyltransferase activity; glycolipid mannosyltransferase activity; inositol phosphoceramide synthase activity; lipopolysaccharide-1,6-galactosyltransferase activity; mannosyltransferase activity; O antigen polymerase activity; oligosaccharyl transferase activity; phenanthrol glycosyltransferase activity; transferase activity; transferase activity, transferring glycosyl groups; UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase activity; UDP-glucose:glycoprotein glucosyltransferase activity

Biological Process: oligosaccharide biosynthetic process; protein amino acid glycosylation