Rabbit PIWI-L1 Polyclonal Antibody | anti-PIWI-L1 antibody

PIWI-L1 Antibody

Cat. Num. AAA9409909

• Gene Names: PIWIL1; HIWI; MIWI; PIWI; CT80.1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: PIWI-L1; Polyclonal Antibody; PIWI-L1 Antibody; PIWIL1; PIWI; MIWI; HIWI; anti-PIWI-L1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 861

• Applicable Applications for anti-PIWI-L1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against an 18 amino acid peptide near the amino terminus of human PIWI-L1.
• Target Name: PIWI-L1
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of PIWI-L1 in HepG2 cell lysate with PIWI-L1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.)

Immunohistochemistry (IHC)

(Immunohistochemistry of MFSD2B in rat liver tissue with MFSD2B antibody at 10 ug/mL.)

Related Product Information for anti-PIWI-L1 antibody

PIWI-L1 is a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that are crucial for the biogenesis and function of small non-coding RNAs and play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. PIWI-L1 is thought to play a role as an intrinsic regulator of the self-renewal capacity of germline and hematopoietic stem cells as removing PIWI-L1 protein from single germline stem cells significantly decreases the rate of their division. Recent studies have shown that overexpression of PIWI-L1 (as well as other PIWI-like proteins) are potential biomarkers for colon and other cancers.

Product Categories/Family for anti-PIWI-L1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 14579645
• NCBI GeneID: 9271
• Molecular Weight: 89,484 Da
• NCBI Official Full Name: PIWI protein
• NCBI Official Synonym Full Names: piwi-like RNA-mediated gene silencing 1
• NCBI Official Symbol: PIWIL1
• NCBI Official Synonym Symbols: HIWI; MIWI; PIWI; CT80.1
• NCBI Protein Information: piwi-like protein 1
• UniProt Protein Name: Piwi-like protein 1
• UniProt Gene Name: PIWIL1
• UniProt Synonym Gene Names: HIWI
• UniProt Entry Name: PIWL1_HUMAN

NCBI Description

This gene encodes a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. The encoded protein may play a role as an intrinsic regulator of the self-renewal capacity of germline and hematopoietic stem cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

PIWIL1: Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer- independent mechanism and are primarily derived from transposons and other repeated sequence elements. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Probable component of some RISC complex, which mediates RNA cleavage and translational silencing. Also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability. Isoform 3 may be a negative developmental regulator. Interacts (via Piwi domain) with DICER1, suggesting that it forms ribonucleoprotein RISC complexes. This interaction is regulated by HSP90AB1 activity. Interacts with MAEL, KIF17, PABPC1, PRMT5 and WDR77. Interacts (when methylated on arginine residues) with TDRD1, TDRKH/TDRD2, RNF17/TDRD4, TDRD6, TDRD7 and TDRD9. Isoform 3 is down-regulated in CD34(+) hematopoietic cells during differentiation. Detected in most fetal and adult tissues. Expressed in testes, specifically in germline cells; detected in spermatocytes and spermatids during spermatogenesis. Increased expression in testicular tumors originating from embryonic germ cells with retention of germ cells phenotype. No expression in testicular tumors of somatic origin, such as Sertoli cell and Leydig cell tumors. Overexpressed in gastric cancer cells. Isoform 3 is ubiquitously expressed, and specifically in CD34+ hematopoietic progenitor cells but not in more differentiated cells. Belongs to the argonaute family. Piwi subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, peripheral

Chromosomal Location of Human Ortholog: 12q24.33

Cellular Component: polysome; mRNA cap complex; cytoplasm

Molecular Function: mRNA binding; protein binding; single-stranded RNA binding; protein kinase binding

Biological Process: regulation of translation; meiotic cell cycle; multicellular organismal development; gene expression; RNA-mediated gene silencing; spermatid development