Rabbit SS18 / SYT Polyclonal Antibody | anti-SS18 antibody

Rabbit Polyclonal (IgG) to Human SS18 / SYT

Cat. Num. AAA243375

• Gene Names: SS18; SYT; SSXT
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: SS18 / SYT; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human SS18 / SYT; Anti-SS18 / SYT Antibody (aa1-50) IHC-plus; SS18; SSXT; SYT; Protein SYT; Hd21; Protein SSXT; Human SS18; anti-SS18 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SSXT Antibody detects endogenous levels of total SSXT protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Sequence Length: 387

• Applicable Applications for anti-SS18 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:1000), IHC-P (5 ug/ml), WB (1:500 - 1:1000)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human SSXT (aa1-50).
• Immunogen: SS18 / SYT antibody was raised against synthetic peptide from human SSXT (aa1-50).
• Antigen Modification: aa1-50
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-SS18 / SYT antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.)

Western Blot (WB)

(Western blot of extracts from HepG2/Jurkat/COLO205 cells, using SSXT Antibody. The lane on the right is treated with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 56117844
• NCBI GeneID: 6760
• NCBI Accession #: NP_005628.2
• NCBI GenBank Nucleotide #: NM_005637.2
• UniProt Accession #: Q15532; Q16404; Q4VAX1; Q9BXC6; B0YJ95
• Molecular Weight: 42,216 Da
• NCBI Official Full Name: protein SSXT isoform 2
• NCBI Official Synonym Full Names: synovial sarcoma translocation, chromosome 18
• NCBI Official Symbol: SS18
• NCBI Official Synonym Symbols: SYT; SSXT
• NCBI Protein Information: protein SSXT; synovial sarcoma, translocated to X chromosome; synovial sarcoma translocated to X chromosome protein
• UniProt Protein Name: Protein SSXT
• Protein Family: Protein
• UniProt Gene Name: SS18
• UniProt Synonym Gene Names: SSXT; SYT
• UniProt Entry Name: SSXT_HUMAN

Uniprot Description

SS18: Appears to function synergistically with RBM14 as a transcriptional coactivator. Isoform 1 and isoform 2 function in nuclear receptor coactivation. Isoform 1 and isoform 2 function in general transcriptional coactivation. A chromosomal aberration involving SS18 may be a cause of synovial sarcoma. Translocation t(X;18)(p11.2;q11.2). The translocation is specifically found in more than 80% of synovial sarcoma. The fusion products SSXT-SSX1 or SSXT-SSX2 are probably responsible for transforming activity. Heterogeneity in the position of the breakpoint can occur (low frequency). Belongs to the SS18 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 18q11.2

Cellular Component: cytoplasmic microtubule; nucleus

Molecular Function: protein binding; ligand-dependent nuclear receptor transcription coactivator activity

Biological Process: response to drug; transcription, DNA-dependent; cell morphogenesis; ephrin receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; microtubule cytoskeleton organization and biogenesis