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Western Blot (WB) (Western blot analysis of SRSF4 expression in HeLa (A), HT1080 (B), NIH3T3 (C) whole cell lysates.)

Rabbit SRSF4 Polyclonal Antibody | anti-SRSF4 antibody

Anti-SRSF4 Antibody

Gene Names
SRSF4; SFRS4; SRP75
Reactivity
Human, Mouse, Rat, Bovine, Pig
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
SRSF4; Polyclonal Antibody; Anti-SRSF4 Antibody; SFRS4; SRP75; Serine/arginine-rich splicing factor 4; Pre-mRNA-splicing factor SRP75; SRP001LB; Splicing factor; arginine/serine-rich 4; anti-SRSF4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Pig
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of SRSF4 protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Sequence Length
494
Applicable Applications for anti-SRSF4 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SRSF4. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of SRSF4 expression in HeLa (A), HT1080 (B), NIH3T3 (C) whole cell lysates.)

Western Blot (WB) (Western blot analysis of SRSF4 expression in HeLa (A), HT1080 (B), NIH3T3 (C) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of SRSF4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of SRSF4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Related Product Information for anti-SRSF4 antibody
Rabbit polyclonal antibody to SRSF4

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
56,678 Da
NCBI Official Full Name
serine/arginine-rich splicing factor 4
NCBI Official Synonym Full Names
serine/arginine-rich splicing factor 4
NCBI Official Symbol
SRSF4
NCBI Official Synonym Symbols
SFRS4; SRP75
NCBI Protein Information
serine/arginine-rich splicing factor 4; SRP001LB; SR splicing factor 4; pre-mRNA-splicing factor SRP75; splicing factor, arginine/serine-rich 4
UniProt Protein Name
Serine/arginine-rich splicing factor 4
UniProt Gene Name
SRSF4
UniProt Synonym Gene Names
SFRS4; SRP75
UniProt Entry Name
SRSF4_HUMAN

NCBI Description

This gene encodes a member of the arginine/serine-rich splicing factor family. The encoded protein likely functions in mRNA processing. [provided by RefSeq, Feb 2009]

Uniprot Description

SFRS4: Plays a role in alternative splice site selection during pre-mRNA splicing. Represses the splicing of MAPT/Tau exon 10. Belongs to the splicing factor SR family.

Protein type: Spliceosome; RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 1p35.3

Cellular Component: nucleoplasm; nuclear speck; nucleus

Molecular Function: nucleotide binding

Biological Process: RNA splicing, via transesterification reactions; nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase II promoter; mRNA export from nucleus; negative regulation of nuclear mRNA splicing, via spliceosome; RNA splicing; hemopoietic progenitor cell differentiation; gene expression; mRNA 3'-end processing; mRNA processing; termination of RNA polymerase II transcription

Research Articles on SRSF4

Similar Products

Product Notes

The SRSF4 srsf4 (Catalog #AAA822269) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SRSF4 Antibody reacts with Human, Mouse, Rat, Bovine, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's SRSF4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB (1/500 - 1/1000), IH (1/100 - 1/200). Researchers should empirically determine the suitability of the SRSF4 srsf4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SRSF4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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