Mouse Serine/Arginine Rich Splicing Factor 4 (SRSF4) ELISA Kit | SRSF4 elisa kit

Mouse Serine/Arginine Rich Splicing Factor 4 (SRSF4) ELISA Kit

Cat. Num. AAA2019207

• Gene Names: SRSF4; SFRS4; SRP75
• Reactivity: Mouse
• Synonyms: Serine/Arginine Rich Splicing Factor 4 (SRSF4); Mouse Serine/Arginine Rich Splicing Factor 4 (SRSF4) ELISA Kit; SFRS4; SRP75; SRP001LB; Pre-mRNA-splicing factor SRP75; Splicing factor; arginine/serine-rich 4; SRSF4 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Serine/Arginine Rich Splicing Factor 4 (SRSF4).; No significant cross-reactivity or interference between Serine/Arginine Rich Splicing Factor 4 (SRSF4) and analogues was observed.
• Sequence Length: 494

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.057ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serine/Arginine Rich Splicing Factor 4 (SRSF4) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serine/Arginine Rich Splicing Factor 4 (SRSF4) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SRSF4 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine/Arginine Rich Splicing Factor 4 (SRSF4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serine/Arginine Rich Splicing Factor 4 (SRSF4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serine/Arginine Rich Splicing Factor 4 (SRSF4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Serine/Arginine Rich Splicing Factor 4 (SRSF4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for SRSF4 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 21361282
• NCBI GeneID: 6429
• NCBI Accession #: NP_005617.2
• NCBI GenBank Nucleotide #: NM_005626.4
• UniProt Accession #: Q08170; Q5VXP1; Q9BUA4; Q9UEB5
• Molecular Weight: 56,678 Da
• NCBI Official Full Name: serine/arginine-rich splicing factor 4
• NCBI Official Synonym Full Names: serine/arginine-rich splicing factor 4
• NCBI Official Symbol: SRSF4
• NCBI Official Synonym Symbols: SFRS4; SRP75
• NCBI Protein Information: serine/arginine-rich splicing factor 4
• UniProt Protein Name: Serine/arginine-rich splicing factor 4
• Protein Family: Serine/arginine-rich splicing factor
• UniProt Gene Name: SRSF4
• UniProt Synonym Gene Names: SFRS4; SRP75
• UniProt Entry Name: SRSF4_HUMAN

NCBI Description

This gene encodes a member of the arginine/serine-rich splicing factor family. The encoded protein likely functions in mRNA processing. [provided by RefSeq, Feb 2009]

Uniprot Description

SFRS4: Plays a role in alternative splice site selection during pre-mRNA splicing. Represses the splicing of MAPT/Tau exon 10. Belongs to the splicing factor SR family.

Protein type: RNA-binding; Spliceosome; RNA splicing

Chromosomal Location of Human Ortholog: 1p35.3

Cellular Component: nucleoplasm; nuclear speck; nucleus

Molecular Function: nucleotide binding

Biological Process: nuclear mRNA splicing, via spliceosome; RNA splicing, via transesterification reactions; transcription from RNA polymerase II promoter; mRNA export from nucleus; negative regulation of nuclear mRNA splicing, via spliceosome; RNA splicing; hemopoietic progenitor cell differentiation; gene expression; mRNA 3'-end processing; mRNA processing; termination of RNA polymerase II transcription