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Western Blot (WB) (Western blot analysis of extracts of SKOV3 cells, using SRSF4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

Rabbit SRSF4 Polyclonal Antibody | anti-SRSF4 antibody

SRSF4 Polyclonal Antibody

Gene Names
SRSF4; SFRS4; SRP75
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Western Blot
Purity
Affinity Purification
Synonyms
SRSF4; Polyclonal Antibody; SRSF4 Polyclonal Antibody; SFRS4; SRP75; serine/arginine-rich splicing factor 4; anti-SRSF4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Concentration
1mg/ml (varies by lot)
Sequence Length
494
Applicable Applications for anti-SRSF4 antibody
Western Blot (WB),Immunofluorescence (IF)
Application Notes
WB: 1:500 - 1:2000
IF: 1:50 - 1:200
Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino accids 1-180 of human SRSF4 (NP_005617.2)
Calculated Molecular Weight
56kDa
Observed Molecular Weight
50KDa
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at -20°C Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of SKOV3 cells, using SRSF4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

Western Blot (WB) (Western blot analysis of extracts of SKOV3 cells, using SRSF4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

Immunofluorescence (IF)

(Immunofluorescence analysis of MCF-7 cells using SRSF4 antibody. Blue: DAPI for nuclear staining.)

Immunofluorescence (IF) (Immunofluorescence analysis of MCF-7 cells using SRSF4 antibody. Blue: DAPI for nuclear staining.)
Related Product Information for anti-SRSF4 antibody
This gene encodes a member of the arginine/serine-rich splicing factor family. The encoded protein likely functions in mRNA processing.
Product Categories/Family for anti-SRSF4 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Serine/arginine-rich splicing factor 4
NCBI Official Synonym Full Names
serine/arginine-rich splicing factor 4
NCBI Official Symbol
SRSF4
NCBI Official Synonym Symbols
SFRS4; SRP75
NCBI Protein Information
serine/arginine-rich splicing factor 4; SRP001LB; SR splicing factor 4; pre-mRNA-splicing factor SRP75; splicing factor, arginine/serine-rich 4
UniProt Protein Name
Serine/arginine-rich splicing factor 4
UniProt Gene Name
SRSF4
UniProt Synonym Gene Names
SFRS4; SRP75
UniProt Entry Name
SRSF4_HUMAN

NCBI Description

This gene encodes a member of the arginine/serine-rich splicing factor family. The encoded protein likely functions in mRNA processing. [provided by RefSeq, Feb 2009]

Uniprot Description

SFRS4: Plays a role in alternative splice site selection during pre-mRNA splicing. Represses the splicing of MAPT/Tau exon 10. Belongs to the splicing factor SR family.

Protein type: RNA splicing; RNA-binding; Spliceosome

Chromosomal Location of Human Ortholog: 1p35.3

Cellular Component: nucleoplasm; nuclear speck; nucleus

Molecular Function: nucleotide binding

Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; RNA splicing, via transesterification reactions; mRNA export from nucleus; negative regulation of nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression; hemopoietic progenitor cell differentiation; mRNA 3'-end processing; mRNA processing; termination of RNA polymerase II transcription

Research Articles on SRSF4

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Product Notes

The SRSF4 srsf4 (Catalog #AAA9126937) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SRSF4 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SRSF4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB),Immunofluorescence (IF). WB: 1:500 - 1:2000 IF: 1:50 - 1:200. Researchers should empirically determine the suitability of the SRSF4 srsf4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SRSF4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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