Rabbit ARF5 Polyclonal Antibody | anti-ARF5 antibody

ARF5 Rabbit Polyclonal

Cat. Num. AAA766765

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: ARF5; Polyclonal Antibody; ARF5 Rabbit Polyclonal; anti-ARF5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 180

• Applicable Applications for anti-ARF5 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen: ADP-ribosylation factor 5
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human breast cancer using MBS766765 (ARF5 antibody) at dilution of 1:50)

Western Blot (WB)

(MCF7 cells were subjected to SDS PAGE followed by western blot with MBS766765 (ARF5 antibody) at dilution of 1:500)

NCBI and Uniprot Product Information

• NCBI GI #: 4502209
• NCBI GeneID: 381
• NCBI Accession #: NP_001653.1
• NCBI GenBank Nucleotide #: NM_001662.3
• Molecular Weight: 20,530 Da
• NCBI Official Full Name: ADP-ribosylation factor 5
• NCBI Official Synonym Full Names: ADP ribosylation factor 5
• NCBI Official Symbol: ARF5
• NCBI Protein Information: ADP-ribosylation factor 5
• UniProt Protein Name: ADP-ribosylation factor 5
• Protein Family: Auxin response factor
• UniProt Gene Name: ARF5
• UniProt Entry Name: ARF5_HUMAN

NCBI Description

This gene is a member of the human ADP-ribosylation factor (ARF) gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. [provided by RefSeq, Dec 2010]

Uniprot Description

ARF5: GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Belongs to the small GTPase superfamily. Arf family.

Protein type: G protein; G protein, monomeric; G protein, monomeric, ARF; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7q31.3

Cellular Component: Golgi apparatus; perinuclear region of cytoplasm; plasma membrane

Molecular Function: GTP binding; GTPase activity; protein binding

Biological Process: protein transport; retrograde vesicle-mediated transport, Golgi to ER; small GTPase mediated signal transduction