Immunohistochemistry (IHC)
(MBS9607007 at 1/100 staining Human brain cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit SRPX Polyclonal Antibody | anti-SRPX antibody
SRPX Antibody
Gene Names
SRPX; DRS; ETX1; SRPX1; HEL-S-83p
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
SRPX; Polyclonal Antibody; SRPX Antibody; Down-regulated by v-SRC; DRS; DRS protein; Drs-1; Drs-2; Epididymis secretory sperm binding protein Li 83p; ETX1; EXT1; HEL S 83p; mDRS-1; mDRS-2; Srpx; SRPX_HUMAN; SRPX1; Sushi repeat containing protein; X chromosome; X linked; Sushi repeat-containing protein SRPX; Sushi-repeat-containing protein; Sushi-repeat-containing protein SRPX; anti-SRPX antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
SRPX antibody detects endogenous levels of total SRPX
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
444
Applicable Applications for anti-SRPX antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human SRPX
Subcellular Location
Cell Surface. Possibly surface of photoreceptor cell.
Tissue Specificity
Retina and heart; less in placenta, pancreas, lung, liver, skeletal muscle, kidney and brain.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog
Similarity
Pig (91%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9607007 at 1/100 staining Human brain cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9607007 at 1/100 staining Human brain cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of HeLa lysate using SRPX antibody. The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of HeLa lysate using SRPX antibody. The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-SRPX antibody
Description: X-linked retinitis pigmentosa (XLRP) is a retinal degeneration disorder. The most common form of XLRP has been localized to the gene locus RP3 by linkage and deletion analysis. RP3 maps to chromosome Xp21.1 between CYBB and OTC. The sushi-repeat-containing protein, x chromosome (SRPX) gene, also designated ETX1, resides within this region and is deleted in XLRP patients. There are at least two splice variants of SRPX, one of which contains a thirty amino acid signal peptide. Both variants contain three complement control protein domains, a hydrophobic region for membrane anchorage, and a cytoplasmic carboxy terminus. SRPX is expressed in retina and heart. SRPX is highly homologous to the drs (downregulated by v-src) human homolog, which suggests a role for SRPX as a tumor suppressor.
Function: May be involved in phagocytosis during disk shedding, cell adhesion to cells other than the pigment epithelium or signal transduction.
Function: May be involved in phagocytosis during disk shedding, cell adhesion to cells other than the pigment epithelium or signal transduction.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 52 kDa
Predicted: 52 kDa
Predicted: 52 kDa
NCBI Official Full Name
sushi repeat-containing protein SRPX isoform 2
NCBI Official Synonym Full Names
sushi repeat containing protein X-linked
NCBI Official Symbol
SRPX
NCBI Official Synonym Symbols
DRS; ETX1; SRPX1; HEL-S-83p
NCBI Protein Information
sushi repeat-containing protein SRPX
UniProt Protein Name
Sushi repeat-containing protein SRPX
Protein Family
UniProt Gene Name
SRPX
UniProt Synonym Gene Names
ETX1
Uniprot Description
May be involved in phagocytosis during disk shedding, cell adhesion to cells other than the pigment epithelium or signal transduction.
Research Articles on SRPX
Similar Products
Product Notes
The SRPX srpx (Catalog #AAA9607007) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SRPX Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SRPX can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the SRPX srpx for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SRPX, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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