Western Blot (WB)
(Western blot analysis SRPK3 using MCF7 whole cell lysates)
Rabbit SRPK3 Polyclonal Antibody | anti-SRPK3 antibody
SRPK3 Antibody
Gene Names
SRPK3; MSSK1; STK23; MSSK-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
SRPK3; Polyclonal Antibody; SRPK3 Antibody; MSSK-1; MSSK1; Muscle specific serine kinase 1; Muscle-specific serine kinase 1; Serine/arginine rich protein specific kinase 3; Serine/arginine-rich protein-specific kinase 3; Serine/threonine protein kinase 23; Serine/threonine protein kinase SRPK3; Serine/threonine-protein kinase 23; SFRS protein kinase 3; SR-protein-specific kinase 3; Srpk3; SRPK3_HUMAN; SRSF protein kinase 3; STK 23; STK23; anti-SRPK3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
SRPK3 antibody detects endogenous levels of total SRPK3
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
566
Applicable Applications for anti-SRPK3 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human SRPK3
Tissue Specificity
Exclusively expressed in skeletal and heart muscle.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog
Similarity
Pig (100%), Bovine (90%), Horse (100%), Sheep (80%), Rabbit (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis SRPK3 using MCF7 whole cell lysates)
Western Blot (WB)
(Western blot analysis SRPK3 using MCF7 whole cell lysates)
Immunofluorescene (IF)
(MBS9609039 staining 293T by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescene (IF)
(MBS9609039 staining 293T by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-SRPK3 antibody
Function: Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains. Phosphorylates the SR splicing factor SRSF1 and the lamin-B receptor (LBR) in vitro. Required for normal muscle development (By similarity).
Similarity: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family.
Similarity: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 58 kDa
Predicted: 63 kDa
Predicted: 63 kDa
NCBI Official Full Name
SRSF protein kinase 3 isoform 2
NCBI Official Synonym Full Names
SRSF protein kinase 3
NCBI Official Symbol
SRPK3
NCBI Official Synonym Symbols
MSSK1; STK23; MSSK-1
NCBI Protein Information
SRSF protein kinase 3
UniProt Protein Name
SRSF protein kinase 3
Protein Family
UniProt Gene Name
SRPK3
UniProt Synonym Gene Names
MSSK1; STK23; MSSK-1; SR-protein-specific kinase 3
NCBI Description
This gene encodes a protein kinase similar to a protein kinase which is specific for the SR (serine/arginine-rich domain) family of splicing factors. A highly similar protein has been shown to play a role in muscle development in mice. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2009]
Uniprot Description
Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains. Phosphorylates the SR splicing factor SRSF1 and the lamin-B receptor (LBR) in vitro. Required for normal muscle development ().
Research Articles on SRPK3
Similar Products
Product Notes
The SRPK3 srpk3 (Catalog #AAA9609039) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SRPK3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SRPK3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:1000-3000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the SRPK3 srpk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SRPK3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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