Rabbit anti-Human, Mouse ST5 Polyclonal Antibody | anti-ST5 antibody

ST5 Antibody

Cat. Num. AAA9411591

• Gene Names: ST5; HTS1; p126; DENND2B
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: ST5; Polyclonal Antibody; ST5 Antibody; Suppression of tumorigenicity 5; HeLa tumor suppression 1; DENN domain-containing protein 2B; DENND2B; anti-ST5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total ST5 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 1137

• Applicable Applications for anti-ST5 antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500~1:3000
• Immunogen Type: Peptide
• Immunogen Description: Synthesized peptide derived from internal of human ST5.
• Target Name: ST5
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts from COLO205 cells, using ST5 antibody.)

Related Product Information for anti-ST5 antibody

Guanine nucleotide exchange factor (GEF) which may activate RAB9A and RAB9B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. May be involved in cytoskeletal organization and tumorogenicity. Isoform 1 seems to be involved in a signaling transduction pathway leading to activation of MAPK1/ERK2. Isoform 3 may block ERK2 activation stimulated by ABL1. Isoform 3 may alter cell morphology and cell growth.Lichy J.H., Nucleic Acids Res. 24:4700-4708(1996).Amid C., Cytogenet. Cell Genet. 93:284-290(2001).Lichy J.H., Cell Growth Differ. 3:541-548(1992).

Product Categories/Family for anti-ST5 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 47132529
• NCBI GeneID: 6764
• NCBI Accession #: NP_005409.3
• NCBI GenBank Nucleotide #: NM_005418.3
• UniProt Accession #: P78524; P78523; Q16492; Q7KYY2; Q7KZ12; Q8NE12; Q9BQQ6; B2R6X7; B3KXQ6
• Molecular Weight: 70,245 Da
• NCBI Official Full Name: suppression of tumorigenicity 5 protein isoform 1
• NCBI Official Synonym Full Names: suppression of tumorigenicity 5
• NCBI Official Symbol: ST5
• NCBI Official Synonym Symbols: HTS1; p126; DENND2B
• NCBI Protein Information: suppression of tumorigenicity 5 protein
• UniProt Protein Name: Suppression of tumorigenicity 5 protein
• Protein Family: Suppression of tumorigenicity 5 protein
• UniProt Gene Name: ST5
• UniProt Synonym Gene Names: DENND2B; HTS1
• UniProt Entry Name: ST5_HUMAN

NCBI Description

This gene was identified by its ability to suppress the tumorigenicity of Hela cells in nude mice. The protein encoded by this gene contains a C-terminal region that shares similarity with the Rab 3 family of small GTP binding proteins. This protein preferentially binds to the SH3 domain of c-Abl kinase, and acts as a regulator of MAPK1/ERK2 kinase, which may contribute to its ability to reduce the tumorigenic phenotype in cells. Three alternatively spliced transcript variants of this gene encoding distinct isoforms are identified. [provided by RefSeq, Jul 2008]

Uniprot Description

DENND2B: a guanine nucleotide exchange factor (GEF) that may activate RAB9A and RAB9B. May be involved in cytoskeletal organization and tumorogenicity. Widely expressed with the exception of peripheral blood lymphocytes and lymphoid cell lines. 3 isoforms of the human protein are produced by alternative promoter. Isoform 1 interacts with the SH3 domain of ABL1, is expressed in several epithelial, fibroblast, and tumorigenic cell lines, and seems to be involved in the activation of ERK2. Isoform 3, expressed in primary cells that may be weakly tumorigenic, but not in tumorigenic cell lines, may block ERK2 activation stimulated by ABL1, and may alter cell morphology and cell growth.

Protein type: GEFs, Rab; GEFs

Chromosomal Location of Human Ortholog: 11p15

Molecular Function: Rab guanyl-nucleotide exchange factor activity