Mouse anti-Human SRPK2 Polyclonal Antibody | anti-SRPK2 antibody

SRPK2 (Serine/Threonine-protein Kinase SRPK2, Serine/Arginine-rich Protein-specific Kinase 2, SR-protein-specific Kinase 2, SFRS Protein Kinase 2, FLJ36101)

Cat. Num. AAA646375

• Gene Names: SRPK2; SFRSK2
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: SRPK2; Polyclonal Antibody; SRPK2 (Serine/Threonine-protein Kinase SRPK2; Serine/Arginine-rich Protein-specific Kinase 2; SR-protein-specific Kinase 2; SFRS Protein Kinase 2; FLJ36101); Anti -SRPK2 (Serine/Threonine-protein Kinase SRPK2; anti-SRPK2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SRPK2.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MSSRKVLAIQARKRRPKREKHPKKPEPQQKAPLVPPPPPPPPPPPPPLPDPTPPEPEEEILGSDDEEQEDPADYCKGGYHPVKIGDLFNGRYHVIRKLGWGHFSTVWLCWDMQGKRFVAMKVVKSAQHYTETALDEIKLLKCVRESDPSDPNKDMVVQLIDDFKISGMNGIHVCMVFEVLGHHLLKWIIKSNYQGLPVRCVKSIIRQVLQGLDYLHSKCKIIHTDIKPENILMCVDDAYVRRMAAEATEWQKAGAPPPSGSAVSTAPQQKPIGKISKNKKKKLKKKQKRQAELLEKRLQEIEELEREAERKIIEENITSAAPSNDQDGEYCPEVKLKTTGLEEAAEAETAKDNGEAEDQEEKEDAEKENIEKDEDDVDQELANIDPTWIESPKTNGHIENGPFSLEQQLDDEDDDEEDCPNPEEYNLDEPNAESDYTYSSSYEQFNGELPNGRHKIPESQFPEFSTSLFSGSLEPVACGSVLSEGSPLTEQEESSPSHDRSRTVSASSTGDLPKAKTRAADLLVNPLDPRNADKIRVKIADLGNACWVHKHFTEDIQTRQYRSIEVLIGAGYSTPADIWSTACMAFELATGDYLFEPHSGEDYSRDEDHIAHIIELLGSIPRHFALSGKYSREFFNRRGELRHITKLKPWSLFDVLVEKYGWPHEDAAQFTDFLIPMLEMVPEKRASAGECLRHPWLNS

• Applicable Applications for anti-SRPK2 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot and Immunofluorescence.; Dilution: Immunofluorescence: 10ug/ml
• Immunogen: Full length human SRPK2, aa1-699 (NP_872634.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of SRPK2 expression in transfected 293T cell line by SRPK2 polyclonal antibody. Lane 1: SRPK2 transfected lysate (76.89kD). Lane 2: Non-transfected lysate.)

Immunofluorescence (IF)

(Immunofluorescence of purified antibody to SRPK2 on HeLa cell. [antibody concentration 10ug/ml].)

Related Product Information for anti-SRPK2 antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

Product Categories/Family for anti-SRPK2 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 506949341
• NCBI GeneID: 6733
• NCBI Accession #: NP_001265202.1
• NCBI GenBank Nucleotide #: NM_001278273.1
• UniProt Accession #: P78362; O75220; O75221; Q6NUL0; Q6V1X2; Q8IYQ3; A8MVX2
• Molecular Weight: 77,527 Da
• NCBI Official Full Name: SRSF protein kinase 2 isoform b
• NCBI Official Synonym Full Names: SRSF protein kinase 2
• NCBI Official Symbol: SRPK2
• NCBI Official Synonym Symbols: SFRSK2
• NCBI Protein Information: SRSF protein kinase 2; SR protein kinase 2; serine kinase SRPK2; SFRS protein kinase 2; SR-protein-specific kinase 2; serine/threonine-protein kinase SRPK2; serine/arginine-rich splicing factor kinase 2; serine/arginine-rich protein-specific kinase 2
• UniProt Protein Name: SRSF protein kinase 2
• Protein Family: SRSF protein kinase
• UniProt Gene Name: SRPK2
• UniProt Synonym Gene Names: SR-protein-specific kinase 2
• UniProt Entry Name: SRPK2_HUMAN

Uniprot Description

Function: Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Promotes neuronal apoptosis by up-regulating cyclin-D1 (CCND1) expression. This is done by the phosphorylation of SRSF2, leading to the suppression of p53/TP53 phosphorylation thereby relieving the repressive effect of p53/TP53 on cyclin-D1 (CCND1) expression. Phosphorylates ACIN1, and redistributes it from the nuclear speckles to the nucleoplasm, resulting in cyclin A1 but not cyclin A2 up-regulation. Plays an essential role in spliceosomal B complex formation via the phosphorylation of DDX23/PRP28. Can mediate hepatitis B virus (HBV) core protein phosphorylation. Plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles. Ref.1 Ref.6 Ref.8 Ref.10 Ref.12 Ref.15 Ref.19 Ref.21

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Cofactor: Magnesium.

Enzyme regulation: Activated by phosphorylation on Ser-52 and Ser-588

By similarity.

Subunit structure: Interacts with PKB/AKT1 in a phosphorylation-dependent manner. The phosphorylated form (by PKB/AKT1) interacts with YWHAB and YWHAE. Interaction with YWHAB suppresses its cleavage by caspases and inhibits the release of its N-terminal pro-apoptotic fragment. Interacts with SFN. Associates with U4/U6-U5 tri-small nuclear ribonucleoproteins (U4/U6-U5 tri-snRNPs). Ref.10 Ref.15

Subcellular location: Cytoplasm. Nucleus. Note: Shuttles between the nucleus and the cytoplasm. KAT5/TIP60 inhibits its nuclear translocation. Phosphorylation at Thr-492 by PKB/AKT1 promotes nuclear translocation. Ref.1 Ref.19 Ref.21

Tissue specificity: Highly expressed in brain, moderately expressed in heart and skeletal muscle and at low levels in lung, liver, and kidney. Ref.1

Post-translational modification: Phosphorylation at Thr-492 by PKB/AKT1 enhances its stimulatory activity in triggering cyclin-D1 (CCND1) expression and promoting apoptosis in neurons, which can be blocked by YWHAB. It also enhances its protein kinase activity toward ACIN1 and SRSF2, promotes its nuclear translocation and prevents its proteolytic cleavage.Proteolytically cleaved at Asp-139 and Asp-403 by caspase-3 during apoptotic cell death. Cleavage at Asp-139 which is the major site of cleavage, produces a small N-terminal fragment that translocates into nucleus and promotes VP16-induced apoptosis. Ref.21

Sequence similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family.Contains 1 protein kinase domain.

Sequence caution: The sequence AAQ63886.1 differs from that shown. Reason: The cDNA appears to contain a duplicated region.