Western Blot (WB)
(Western blot analysis of PTPN5 in Jurkat cell lysate with PTPN5 antibody at (A) 1 and (B) 2 μg/ml.)
Rabbit anti-Human, Mouse PTPN5 Polyclonal Antibody | anti-PTPN5 antibody
PTPN5 Antibody
Gene Names
PTPN5; STEP; PTPSTEP
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
PTPN5 antibody is affinity chromatography purified via peptide column.
Synonyms
PTPN5; Polyclonal Antibody; PTPN5 Antibody; Tyrosine-protein phosphatase non-receptor type 5; Neural-specific protein-tyrosine phosphatase; PTPSTEP; Striatum-enriched protein-tyrosine phosphatase; STEP ; protein tyrosine phosphatase; non-receptor type 5 (striatum-enriched); anti-PTPN5 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
PTPN5 antibody is human and mouse reactive.
Purity/Purification
PTPN5 antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
565
Applicable Applications for anti-PTPN5 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
PTPN5 antibody can be used for detection of PTPN5 by Western blot at 1 - 2 mug/ml. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
PTPN5 antibody was raised against an 18 amino acid peptide near the amino terminus of human PTPN5.
Buffer
PTPN5 antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
PTPN5 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
Western Blot (WB)
(Western blot analysis of PTPN5 in Jurkat cell lysate with PTPN5 antibody at (A) 1 and (B) 2 μg/ml.)
Western Blot (WB)
(Western blot analysis of PTPN5 in Jurkat cell lysate with PTPN5 antibody at (A) 1 and (B) 2 μg/ml.)
Immunohistochemistry (IHC)
(Immunohistochemistry of PTPN5 in mouse brain tissue with PTPN5 antibody at 5 μg/ml.)
Immunohistochemistry (IHC)
(Immunohistochemistry of PTPN5 in mouse brain tissue with PTPN5 antibody at 5 μg/ml.)
Immunofluorescence (IF)
(Immunofluorescence of PTPN5 in mouse brain tissue with PTPN5 antibody at 20 μg/ml.)
Immunofluorescence (IF)
(Immunofluorescence of PTPN5 in mouse brain tissue with PTPN5 antibody at 20 μg/ml.)
Related Product Information for anti-PTPN5 antibody
The protein tyrosine phosphatase PTPN5, also known as Striatal enriched phosphatase (STEP), is involved in the regulation of synaptic plasticity and neuronal cell survival, including MAPKs, Src family kinases and NMDA receptors (1). It is expressed in dopaminoceptive neurons of the central nervous system and multiple forms of PTPN5 show differential enrichment in adult brain regions (2). NMDA-mediated activation of PTPN5 is an important mechanism for regulation of Erk activity in neurons (3). Furthermore, PTPN5 is involved in the regulation of both NMDAR and AMPAR trafficking (4,5). PTPN5 may play a role in Alzheimer's disease (1).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 62 kDa
Observed: 60 kDa
Observed: 60 kDa
NCBI Official Full Name
tyrosine-protein phosphatase non-receptor type 5 isoform a
NCBI Official Synonym Full Names
protein tyrosine phosphatase, non-receptor type 5 (striatum-enriched)
NCBI Official Symbol
PTPN5
NCBI Official Synonym Symbols
STEP; PTPSTEP
NCBI Protein Information
tyrosine-protein phosphatase non-receptor type 5; neural-specific protein-tyrosine phosphatase; protein-tyrosine phosphatase striatum-enriched; striatum-enriched protein-tyrosine phosphatase
UniProt Protein Name
Tyrosine-protein phosphatase non-receptor type 5
Protein Family
UniProt Gene Name
PTPN5
UniProt Synonym Gene Names
STEP
UniProt Entry Name
PTN5_HUMAN
Uniprot Description
STEP: a non-receptor protein-tyrosine phosphatase. STEP (striatum enriched phosphatase) Contains a single phosphatase catalytic domain located at the C-terminus. Two alternatively-spliced messages have been described.
Protein type: Motility/polarity/chemotaxis; EC 3.1.3.48; Protein phosphatase, tyrosine (non-receptor); Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 11p15.1
Cellular Component: endoplasmic reticulum membrane; integral to membrane
Molecular Function: phosphotyrosine binding; protein tyrosine phosphatase activity
Biological Process: protein amino acid dephosphorylation
Research Articles on PTPN5
Similar Products
Product Notes
The PTPN5 ptpn5 (Catalog #AAA153395) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PTPN5 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PTPN5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). PTPN5 antibody can be used for detection of PTPN5 by Western blot at 1 - 2 mug/ml. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the PTPN5 ptpn5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PTPN5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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