Rabbit anti-Human SRPK1 Polyclonal Antibody | anti-SRPK1 antibody

SRPK1 (SFRS Protein Kinase 1, SR Protein Kinase 1, SFRSK1) (APC)

Cat. Num. AAA6438894

• Gene Names: SRPK1; SFRSK1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: SRPK1; Polyclonal Antibody; SRPK1 (SFRS Protein Kinase 1; SR Protein Kinase 1; SFRSK1) (APC); anti-SRPK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SRPK1.
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-SRPK1 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:200; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human SRPK1.
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using SRPK1 antibody.)

Product Categories/Family for anti-SRPK1 antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 47419936
• NCBI GeneID: 6732
• NCBI Accession #: NP_003128.3
• NCBI GenBank Nucleotide #: NM_003137.4
• UniProt Accession #: Q96SB4; Q12890; Q5R364; Q5R365; Q8IY12
• Molecular Weight: 74,325 Da
• NCBI Official Full Name: SRSF protein kinase 1
• NCBI Official Synonym Full Names: SRSF protein kinase 1
• NCBI Official Symbol: SRPK1
• NCBI Official Synonym Symbols: SFRSK1
• NCBI Protein Information: SRSF protein kinase 1; SFRS protein kinase 1; SR-protein-specific kinase 1; serine/threonine-protein kinase SRPK1; serine/arginine-rich splicing factor kinase 1
• UniProt Protein Name: SRSF protein kinase 1
• Protein Family: SRSF protein kinase
• UniProt Gene Name: SRPK1
• UniProt Synonym Gene Names: SR-protein-specific kinase 1
• UniProt Entry Name: SRPK1_HUMAN

NCBI Description

This gene encodes a serine/arginine protein kinase specific for the SR (serine/arginine-rich domain) family of splicing factors. The protein localizes to the nucleus and the cytoplasm. It is thought to play a role in regulation of both constitutive and alternative splicing by regulating intracellular localization of splicing factors. Alternative splicing of this gene results in multiple transcript variants. Additional alternatively spliced transcript variants have been described for this gene, but their full length nature have not been determined.[provided by RefSeq, Jul 2010]

Uniprot Description

SRPK1: Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Plays a central role in the regulatory network for splicing, controlling the intranuclear distribution of splicing factors in interphase cells and the reorganization of nuclear speckles during mitosis. Can influence additional steps of mRNA maturation, as well as other cellular activities, such as chromatin reorganization in somatic and sperm cells and cell cycle progression. Isoform 2 phosphorylates SFRS2, ZRSR2, LBR and PRM1. Isoform 2 phosphorylates SRSF1 using a directional (C-terminal to N-terminal) and a dual-track mechanism incorporating both processive phosphorylation (in which the kinase stays attached to the substrate after each round of phosphorylation) and distributive phosphorylation steps (in which the kinase and substrate dissociate after each phosphorylation event). The RS domain of SRSF1 binds first to a docking groove in the large lobe of the kinase domain of SRPK1. This induces certain structural changes in SRPK1 and/or RRM2 domain of SRSF1, allowing RRM2 to bind the kinase and initiate phosphorylation. The cycles continue for several phosphorylation steps in a processive manner (steps 1-8) until the last few phosphorylation steps (approximately steps 9-12). During that time, a mechanical stress induces the unfolding of the beta-4 motif in RRM2, which then docks at the docking groove of SRPK1. This also signals RRM2 to begin to dissociate, which facilitates SRSF1 dissociation after phosphorylation is completed. Isoform 2 can mediate hepatitis B virus (HBV) core protein phosphorylation. It plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles. Isoform 1 and isoform 2 can induce splicing of exon 10 in MAPT/TAU. The ratio of isoform 1/isoform 2 plays a decisive role in determining cell fate in K562 leukaemic cell line: isoform 2 favors proliferation where as isoform 1 favors differentiation. Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, CMGC; CMGC group; SRPK family

Chromosomal Location of Human Ortholog: 6p21.31

Cellular Component: nucleoplasm; nuclear matrix; endoplasmic reticulum; cytoplasm; plasma membrane; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; magnesium ion binding; ATP binding; protein kinase activity

Biological Process: regulation of nuclear mRNA splicing, via spliceosome; positive regulation of viral genome replication; sperm chromatin condensation; viral reproduction; negative regulation of viral genome replication; RNA splicing; innate immune response; mRNA processing; protein amino acid phosphorylation; chromosome segregation; regulation of mRNA processing