Rabbit anti-Human CCM2 Polyclonal Antibody | anti-CCM2 antibody

CCM2 (Malcavernin, Cerebral Cavernous Malformations 2 Protein, C7orf22, PP10187, MGC4067, MGC4607, MGC74868) (AP)

Cat. Num. AAA6372630

• Gene Names: CCM2; OSM; C7orf22; PP10187
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CCM2; Polyclonal Antibody; CCM2 (Malcavernin; Cerebral Cavernous Malformations 2 Protein; C7orf22; PP10187; MGC4067; MGC4607; MGC74868) (AP); anti-CCM2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CCM2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-CCM2 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human CCM2, aa1-444 (NP_113631.1).
• Immunogen Sequence: MEEEGKKGKKPGIVSPFKRVFLKGEKSRDKKAHEKVTERRPLHTVVLSLPERVEPDRLLSDYIEKEVKYLGQLTSIPGYLNPSSRTEILHFIDNAKRAHQLPGHLTQEHDAVLSLSAYNVKLAWRDGEDIILRVPIHDIAAVSYVRDDAAHLVVLKTAQDPGISPSQSLCAESSRGLSAGSLSESAVGPVEACCLVILAAESKVAAEELCCLLGQVFQVVYTESTIDFLDRAIFDGASTPTHHLSLHSDDSSTKVDIKETYEVEASTFCFPESVDVGGASPHSKTISESELSASATELLQDYMLTLRTKLSSQEIQQFAALLHEYRNGASIHEFCINLRQLYGDSRKFLLLGLRPFIPEKDSQHFENFLETIGVKDGRGIITDSFGRHRRALSTTSSSTTNGNRATGSSDDRSAPSEGDEWDRMISDISSDIEALGCSMDQDSA
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of CCM2 expression in transfected 293T cell line by CCM2 polyclonal antibody. Lane 1: CCM2 transfected lysate (48.8kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-CCM2 antibody

CCM2 is a scaffold protein that functions in the stress-activated p38 Mitogen-activated protein kinase (MAPK) signaling cascade. The protein interacts with SMAD specific E3 ubiquitin protein ligase 1 (also known as SMURF1) via a phosphotyrosine binding domain to promote RhoA degradation. The protein is required for normal cytoskeletal structure, cell-cell interactions, and lumen formation in endothelial cells.

Product Categories/Family for anti-CCM2 antibody

Antibodies; KIAA; orf

NCBI and Uniprot Product Information

• NCBI GI #: 13899275
• NCBI GeneID: 83605
• NCBI Accession #: NP_113631.1
• NCBI GenBank Nucleotide #: NM_031443.3
• UniProt Accession #: Q9BSQ5; Q71RE5; Q8TAT4; A4D2L4; B3KUV0; D3DVL4; E9PDJ3; F5H0E1; F5H551
• Molecular Weight: 42,073 Da
• NCBI Official Full Name: cerebral cavernous malformations 2 protein isoform 2
• NCBI Official Synonym Full Names: CCM2 scaffold protein
• NCBI Official Symbol: CCM2
• NCBI Official Synonym Symbols: OSM; C7orf22; PP10187
• NCBI Protein Information: cerebral cavernous malformations 2 protein
• UniProt Protein Name: Malcavernin
• Protein Family: Cerebral cavernous malformations protein
• UniProt Gene Name: CCM2
• UniProt Synonym Gene Names: C7orf22
• UniProt Entry Name: CCM2_HUMAN

NCBI Description

This gene encodes a scaffold protein that functions in the stress-activated p38 Mitogen-activated protein kinase (MAPK) signaling cascade. The protein interacts with SMAD specific E3 ubiquitin protein ligase 1 (also known as SMURF1) via a phosphotyrosine binding domain to promote RhoA degradation. The protein is required for normal cytoskeletal structure, cell-cell interactions, and lumen formation in endothelial cells. Mutations in this gene result in cerebral cavernous malformations. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Nov 2009]

Uniprot Description

CCM2: Component of the CCM signaling pathway which is a crucial regulator of heart and vessel formation and integrity. May act through the stabilization of endothelial cell junctions. May function as a scaffold protein for MAP2K3-MAP3K3 signaling. Seems to play a major role in the modulation of MAP3K3- dependent p38 activation induced by hyperosmotic shock. Defects in CCM2 are the cause of cerebral cavernous malformations type 2 (CCM2). Cerebral cavernous malformations (CCMs) are congenital vascular anomalies of the central nervous system that can result in hemorrhagic stroke, seizures, recurrent headaches, and focal neurologic deficits. CCMs have an incidence of 0.1%-0.5% in the general population and are usually present clinically during the 3rd to 5th decades of life. The lesions are characterized by grossly enlarged blood vessels consisting of a single layer of endothelium and without any intervening neural tissue, ranging in diameter from a few millimeters to several centimeters. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7p13

Cellular Component: protein complex; cytoplasm

Molecular Function: protein binding

Biological Process: integrin-mediated signaling pathway; intercellular junction assembly and maintenance; pericardium development; venous blood vessel morphogenesis; in utero embryonic development; multicellular organism growth; stress-activated MAPK cascade; vasculogenesis; inner ear development; endothelial cell development

Disease: Cerebral Cavernous Malformations 2