Western Blot (WB)
(Western Blot analysis of SRSF4 antibody.)
Rabbit SRp75 Polyclonal Antibody | anti-SRp75 antibody
SRp75 Polyclonal Antibody
Gene Names
SRSF4; SFRS4; SRP75
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
SRp75; Polyclonal Antibody; SRp75 Polyclonal Antibody; arginine/serine-rich 4; Pre mRNA splicing factor SRP75; Pre-mRNA-splicing factor SRP75; Serine/arginine-rich splicing factor 4; Splicing factor; arginine/serine rich 4; SRP001LB; SRP75; Srsf4; anti-SRp75 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
SRp75 polyclonal antibody detects endogenous levels of SRp75 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
494
Applicable Applications for anti-SRp75 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IF: 1:50-1:100
IF: 1:50-1:100
Immunogen
Recombinant full length Human SRp75.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot analysis of SRSF4 antibody.)
Western Blot (WB)
(Western Blot analysis of SRSF4 antibody.)
Immunofluorescence (IF)
(Immunofluorescence analysis of MCF7 cell using SRSF4 antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of MCF7 cell using SRSF4 antibody. Blue: DAPI for nuclear staining.)
Related Product Information for anti-SRp75 antibody
Serine/arginine-rich protein 75 (SRp75) is encoded by the SRSF4 gene. SRp75 belongs to the arginine/serine-rich factor family and likely plays a role in alternative mRNA splicing.The family of SR factors all contain one or more RNA recognition motifs (RRM) and an arginine/ serine (RS)-rich domain. They are not only essential for constitutive splicing but also regulate splicing in a concentration-dependent manner by influencing the selection of alternative splice sites. The majority of SR proteins, including SC35 and SRp40, are confined to the nucleus, while SF2/ASF, SRp20, and 9G8 are continuously shuttled between the nucleus and the cytoplasm and contribute to mRNA transport. The activity of SR proteins in regulated splicing is antagonized by members of the hnRNP A/B family of proteins, which induce drastic shifts in the selection of splicing sites.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 50kDa
NCBI Official Full Name
serine/arginine-rich splicing factor 4
NCBI Official Synonym Full Names
serine and arginine rich splicing factor 4
NCBI Official Symbol
SRSF4
NCBI Official Synonym Symbols
SFRS4; SRP75
NCBI Protein Information
serine/arginine-rich splicing factor 4
UniProt Protein Name
Serine/arginine-rich splicing factor 4
UniProt Gene Name
SRSF4
UniProt Synonym Gene Names
SFRS4; SRP75
UniProt Entry Name
SRSF4_HUMAN
NCBI Description
This gene encodes a member of the arginine/serine-rich splicing factor family. The encoded protein likely functions in mRNA processing. [provided by RefSeq, Feb 2009]
Uniprot Description
SFRS4: Plays a role in alternative splice site selection during pre-mRNA splicing. Represses the splicing of MAPT/Tau exon 10. Belongs to the splicing factor SR family.
Protein type: Spliceosome; RNA splicing; RNA-binding
Chromosomal Location of Human Ortholog: 1p35.3
Cellular Component: nucleoplasm; nuclear speck; nucleus
Molecular Function: nucleotide binding
Biological Process: RNA splicing, via transesterification reactions; nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase II promoter; mRNA export from nucleus; negative regulation of nuclear mRNA splicing, via spliceosome; RNA splicing; hemopoietic progenitor cell differentiation; gene expression; mRNA 3'-end processing; mRNA processing; termination of RNA polymerase II transcription
Research Articles on SRp75
Similar Products
Product Notes
The SRp75 srsf4 (Catalog #AAA3011875) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SRp75 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SRp75 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). WB: 1:500-1:2000 IF: 1:50-1:100. Researchers should empirically determine the suitability of the SRp75 srsf4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SRp75, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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