Rabbit anti-Human SRP72 Polyclonal Antibody | anti-SRP72 antibody

SRP72, ID (Signal Recognition Particle 72kD Protein) (PE)

Cat. Num. AAA6499522

• Gene Names: SRP72; BMFF; BMFS1; HEL103
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: SRP72; Polyclonal Antibody; ID (Signal Recognition Particle 72kD Protein) (PE); anti-SRP72 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SRP72.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 671

• Applicable Applications for anti-SRP72 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human SRP72 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of hela cells using SRP72 Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot analysis of SRP72 Antibody (Center) in Hela cell line lysates (35ug/lane). SRP72 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human pancreas with SRP72 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Immunofluorescence analysis of SRP72 Antibody (Center) with hela cells. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.)

Product Categories/Family for anti-SRP72 antibody

Antibodies; Proteins

References

1. Utz, P.J., et.al., J. Biol. Chem. 273 (52), 35362-35370 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 109638749
• NCBI GeneID: 6731
• NCBI Accession #: NP_008878.3
• NCBI GenBank Nucleotide #: NP_008878.3
• UniProt Accession #: O76094
• Molecular Weight: 74.606
• NCBI Official Full Name: signal recognition particle subunit SRP72 isoform 1
• NCBI Official Synonym Full Names: signal recognition particle 72
• NCBI Official Symbol: SRP72
• NCBI Official Synonym Symbols: BMFF; BMFS1; HEL103
• NCBI Protein Information: signal recognition particle subunit SRP72
• UniProt Protein Name: Signal recognition particle subunit SRP72
• Protein Family: Signal recognition particle
• UniProt Gene Name: SRP72
• UniProt Synonym Gene Names: SRP72
• UniProt Entry Name: SRP72_HUMAN

NCBI Description

This gene encodes the 72 kDa subunit of the signal recognition particle (SRP), a ribonucleoprotein complex that mediates the targeting of secretory proteins to the endoplasmic reticulum (ER). The SRP complex consists of a 7S RNA and 6 protein subunits: SRP9, SRP14, SRP19, SRP54, SRP68, and SRP72, that are bound to the 7S RNA as monomers or heterodimers. SRP has at least 3 distinct functions that can be associated with the protein subunits: signal recognition, translational arrest, and ER membrane targeting by interaction with the docking protein. Mutations in this gene are associated with familial bone marrow failure. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]