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Western Blot (WB) (Ufc1 Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the Ufc1 antibody detected the Ufc1 protein (arrow).)

Rabbit anti-Human, mouse Ufc1 Polyclonal Antibody | anti-UFC1 antibody

Ufc1 Antibody (C-term)

Gene Names
UFC1; HSPC155
Reactivity
Human, mouse
Applications
Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Ufc1; Polyclonal Antibody; Ufc1 Antibody (C-term); Ubiquitin-fold modifier-conjugating enzyme 1; Ufm1-conjugating enzyme 1; UFC1; anti-UFC1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Ufc1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human Ufc1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
167
Applicable Applications for anti-UFC1 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Ufc1 Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the Ufc1 antibody detected the Ufc1 protein (arrow).)

Western Blot (WB) (Ufc1 Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the Ufc1 antibody detected the Ufc1 protein (arrow).)

Western Blot (WB)

(Western blot analysis of Ufc1 (arrow) using rabbit polyclonal Ufc1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Ufc1 gene.)

Western Blot (WB) (Western blot analysis of Ufc1 (arrow) using rabbit polyclonal Ufc1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Ufc1 gene.)

Immunohistochemistry (IHC)

(Ufc1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Ufc1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Ufc1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Ufc1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Ufc1 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (Ufc1 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-UFC1 antibody
Various ubiquitin-like (UBL) post-translational modifiers contribute critically to the protein degradation pathway. These UBLs are covalently linked to most target proteins through an enzymatic cascade analogous to ubiquitylation, consisting of E1 (activating), E2 (conjugating), and E3 (ligating) enzymes. Ufc1 Belongs to the ubiquitin-conjugating enzyme family, and is an E2-like enzyme which forms an intermediate with UFM1, a novel ubiquitin-fold modifier, via thioester linkage.
Product Categories/Family for anti-UFC1 antibody
References
Komatsu, M., et al., EMBO J. 23(9):1977-1986 (2004).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
19458
NCBI Official Full Name
ubiquitin-fold modifier-conjugating enzyme 1
NCBI Official Synonym Full Names
ubiquitin-fold modifier conjugating enzyme 1
NCBI Official Symbol
UFC1
NCBI Official Synonym Symbols
HSPC155
NCBI Protein Information
ubiquitin-fold modifier-conjugating enzyme 1
UniProt Protein Name
Ubiquitin-fold modifier-conjugating enzyme 1
UniProt Gene Name
UFC1
UniProt Synonym Gene Names
Ufm1-conjugating enzyme 1
UniProt Entry Name
UFC1_HUMAN

NCBI Description

UFC1 is an E2-like conjugating enzyme for ubiquitin-fold modifier-1 (UFM1; MIM 610553) (Komatsu et al., 2004 [PubMed 15071506]).[supplied by OMIM, Mar 2008]

Uniprot Description

UFC1: E2-like enzyme which forms an intermediate with UFM1 via a thioester linkage. Belongs to the ubiquitin-conjugating enzyme family. UFC1 subfamily.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 1q23.3

Molecular Function: protein binding

Research Articles on UFC1

Similar Products

Product Notes

The UFC1 ufc1 (Catalog #AAA9209279) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ufc1 Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Ufc1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the UFC1 ufc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ufc1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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