Rabbit anti-Human SRP14 Polyclonal Antibody | anti-SRP14 antibody

SRP14, ID (SRP14, Signal recognition particle 14kD protein, 18kD Alu RNA-binding protein) (MaxLight 550)

Cat. Num. AAA6351176

• Gene Names: SRP14; ALURBP
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: SRP14; Polyclonal Antibody; ID (SRP14; Signal recognition particle 14kD protein; 18kD Alu RNA-binding protein) (MaxLight 550); 18kD Alu RNA-binding protein; anti-SRP14 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SRP14
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-SRP14 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 28-55 in the central terminal region of human SRP14, UniProt Accession #P37108; Q4R5C7; NP_003125.3.
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in A375 cell line lysates (35ug/lane) usingMBS6005444. This demonstrates that MBS6005444 detected the SRP14 protein (arrow).)

Related Product Information for anti-SRP14 antibody

Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP9 together with SRP14 and the Alu portion of the SRP RNA, constitutes the elongation arrest domain of SRP. The complex of SRP9 and SRP14 is required for SRP RNA binding.

Product Categories/Family for anti-SRP14 antibody

Antibodies; Binding Proteins

References

1. Mary, C., et al. RNA 16(5):969-979(2010). 2. Lakkaraju, A.K., et al. Cell 133(3):440-451(2008). 3. Yoshida, M., et al. Mol. Pharmacol. 73(3):987-994(2008). 4. Lamesch, P., et al. Genomics 89(3):307-315(2007). 5. Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 149999611
• NCBI GeneID: 6727
• NCBI Accession #: NP_003125.3
• NCBI GenBank Nucleotide #: NM_003134.4
• UniProt Accession #: P37108; Q4R5C7
• Molecular Weight: 14,570 Da
• NCBI Official Full Name: signal recognition particle 14 kDa protein
• NCBI Official Synonym Full Names: signal recognition particle 14kDa (homologous Alu RNA binding protein)
• NCBI Official Symbol: SRP14
• NCBI Official Synonym Symbols: ALURBP
• NCBI Protein Information: signal recognition particle 14 kDa protein; 18 kDa Alu RNA-binding protein
• UniProt Protein Name: Signal recognition particle 14 kDa protein
• Protein Family: Signal recognition particle
• UniProt Gene Name: SRP14
• UniProt Synonym Gene Names: SRP14
• UniProt Entry Name: SRP14_HUMAN

Uniprot Description

SRP14: Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP9 together with SRP14 and the Alu portion of the SRP RNA, constitutes the elongation arrest domain of SRP. The complex of SRP9 and SRP14 is required for SRP RNA binding. Belongs to the SRP14 family.

Protein type: RNA-binding; Translation

Chromosomal Location of Human Ortholog: 15q22

Cellular Component: intercellular bridge; cytoplasm; nucleolus; signal recognition particle, endoplasmic reticulum targeting; cytosol; nucleus

Molecular Function: protein binding; RNA binding; endoplasmic reticulum signal peptide binding; 7S RNA binding

Biological Process: response to drug; SRP-dependent cotranslational protein targeting to membrane; protein targeting to ER; cellular protein metabolic process; translation; gene expression; cotranslational protein targeting to membrane