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Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using SRP14 antibody.)
Rabbit anti-Human, Rat SRP14 Polyclonal Antibody | anti-SRP14 antibody
SRP14 Antibody
Gene Names
SRP14; ALURBP
Reactivity
Human, Rat
Purity
Peptide affinity purification
Synonyms
SRP14; Polyclonal Antibody; SRP14 Antibody; 18 kDa Alu RNA binding protein; 18 kDa Alu RNA-binding protein; Alu RNA-binding protein; 14-KD subunit; ALURBP; MGC14326; Signal recognition particle 14 kDa protein; signal recognition particle 14kDa (homologous Alu RNA binding protein); SRP 14; SRP14_HUMAN; anti-SRP14 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Specificity
SRP14 Antibody detects endogenous levels of total SRP14.
Purity/Purification
Peptide affinity purification
Form/Format
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Application Notes
WB 1:500-1:2000
IHC 1:50-1:200
IF/ICC 1:100-1:500,
ELISA(peptide) 1:20000-1:40000
IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
IHC 1:50-1:200
IF/ICC 1:100-1:500,
ELISA(peptide) 1:20000-1:40000
IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Immunogen
A synthesized peptide derived from human SRP14, corresponding to a region within the internal amino acids.
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using SRP14 antibody.)
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using SRP14 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from Hela, using SRP14 antibody. Lane 1 was treated with the blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Hela, using SRP14 antibody. Lane 1 was treated with the blocking peptide.)
Immunohistochemistry (IHC)
(MBS9603551 at 1/100 staining Human lymph cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9603551 at 1/100 staining Human lymph cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunofluorescence (IF)
(MBS9603551 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9603551 1:200) and mouse antibeta tubulin Ab(MBS9610328 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9603551 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9603551 1:200) and mouse antibeta tubulin Ab(MBS9610328 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.)
Related Product Information for anti-SRP14 antibody
Function: Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP9 together with SRP14 and the Alu portion of the SRP RNA, constitutes the elongation arrest domain of SRP. The complex of SRP9 and SRP14 is required for SRP RNA binding.
Subunit Structure: Signal recognition particle consists of a 7S RNA molecule of 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19, SRP14 and SRP9.
Similarity: Belongs to the SRP14 family.
Subunit Structure: Signal recognition particle consists of a 7S RNA molecule of 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19, SRP14 and SRP9.
Similarity: Belongs to the SRP14 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18 kDa
NCBI Official Full Name
signal recognition particle 14 kDa protein isoform 1
NCBI Official Synonym Full Names
signal recognition particle 14
NCBI Official Symbol
SRP14
NCBI Official Synonym Symbols
ALURBP
NCBI Protein Information
signal recognition particle 14 kDa protein
UniProt Protein Name
Signal recognition particle 14 kDa protein
Protein Family
UniProt Gene Name
SRP14
UniProt Synonym Gene Names
SRP14
Uniprot Description
Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP9 together with SRP14 and the Alu portion of the SRP RNA, constitutes the elongation arrest domain of SRP. The complex of SRP9 and SRP14 is required for SRP RNA binding.
Research Articles on SRP14
Similar Products
Product Notes
The SRP14 srp14 (Catalog #AAA9603551) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SRP14 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. WB 1:500-1:2000 IHC 1:50-1:200 IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000 IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight. Researchers should empirically determine the suitability of the SRP14 srp14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SRP14, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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