Rabbit anti-Human, Mouse SRF Polyclonal Antibody | anti-SRF antibody

Phospho-SRF (Ser77) Antibody

Cat. Num. AAA7133449

• Gene Names: SRF; MCM1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin
• Synonyms: SRF; Polyclonal Antibody; Phospho-SRF (Ser77) Antibody; serum response factor; anti-SRF antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin
• Form/Format: Liquid; Rabbit IgG In Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol.

• Applicable Applications for anti-SRF antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:3000; IHC: 1:50-1:100
• Immunogen: Peptide sequence around phosphorylation site of serine 77 (L-Y-S(p)-G-S) derived from Human SRF.
• Immunogen Species: Human
• Conjugate: Non-conjugated
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blot analysis of extracts from Jurkat cells, treated with PMA (125ng/ml, 30mins), using SRF (Phospho-Ser77) antibody. The lane on the right is treated with the synthesized peptide.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human brain tissue using SRF (Phospho-Ser77) antibody. The picture on the right is treated with the synthesized peptide.)

Product Categories/Family for anti-SRF antibody

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 4507205
• NCBI GeneID: 6722
• NCBI Accession #: NP_003122.1
• NCBI GenBank Nucleotide #: NM_003131.2
• UniProt Accession #: P11831; Q5T648
• Molecular Weight: 51,593 Da
• NCBI Official Full Name: serum response factor
• NCBI Official Synonym Full Names: serum response factor (c-fos serum response element-binding transcription factor)
• NCBI Official Symbol: SRF
• NCBI Official Synonym Symbols: MCM1
• NCBI Protein Information: serum response factor
• UniProt Protein Name: Serum response factor
• Protein Family: Serum response factor
• UniProt Gene Name: SRF
• UniProt Synonym Gene Names: SRF
• UniProt Entry Name: SRF_HUMAN

NCBI Description

This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs). [provided by RefSeq, Jul 2008]

Uniprot Description

SRF: a transcription factor of the MADS domain family that binds to the serum response element (SRE). Regulates the transcription of immediate early genes including c-fos. Binds DNA as a multimer, probably a dimer.

Protein type: Transcription factor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: nuclear chromatin; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein homodimerization activity; chromatin DNA binding; transcription factor binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; developmental growth; erythrocyte development; positive regulation of transcription by glucose; cell-matrix adhesion; response to toxin; heart development; positive regulation of axon extension; response to hormone stimulus; neuron migration; cardiac myofibril assembly; muscle maintenance; negative regulation of cell proliferation; tangential migration from the subventricular zone to the olfactory bulb; regulation of smooth muscle cell differentiation; morphogenesis of an epithelial sheet; positive regulation of smooth muscle contraction; heart looping; negative regulation of cell migration; neurite development; associative learning; positive thymic T cell selection; positive regulation of filopodium formation; regulation of cell adhesion; platelet activation; skin morphogenesis; hippocampus development; sarcomere organization; angiogenesis involved in wound healing; mRNA transcription from RNA polymerase II promoter; trophectodermal cell differentiation; regulation of water loss via skin; patterning of blood vessels; contractile actin filament bundle formation; mesoderm formation; long-term memory; cell migration during sprouting angiogenesis; response to cytokine stimulus; stress fiber formation; response to hypoxia; neuron development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; platelet formation; positive regulation of cell differentiation