Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Mothers against decapentaplegic homolog 9 (SMAD9) Standard Curve.)
Human Mothers Against Decapentaplegic Homolog 9 (SMAD9) ELISA Kit | SMAD9 elisa kit
Human Mothers Against Decapentaplegic Homolog 9 (SMAD9) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Mothers against decapentaplegic homolog 9 (SMAD9) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Mothers against decapentaplegic homolog 9 (SMAD9) Standard Curve.)
Background: SMAD9 or Mothers against decapentaplegic homolog 9 is a polypeptide that, as its name describes, is a homolog of the Drosophila gene: "Mothers against decapentaplegic". It belongs to the SMAD family of proteins, which belong to the TGFbeta superfamily of modulators. Like many other TGFbeta family members, SMAD9 is involved in cell signalling. When a bone morphogenetic protein binds to a receptor (BMP type 1 receptor kinase) it causes SMAD9 to interact with SMAD anchor for receptor activation (SARA).The binding of ligands causes the phosphorylation of the SMAD9 protein and the dissociation from SARA and the association with SMAD4. It is subsequently transferred to the nucleus where it forms complexes with other proteins and acts as a transcription factor
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the SMAD family, which transduces signals from TGF-beta family members. The encoded protein is activated by bone morphogenetic proteins and interacts with SMAD4. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2010]
Uniprot Description
Smad8: transcription factor phosphorylated and activated by bone morphogenetic protein receptor kinases. Phosphorylated Smads dimerize with collaborating Smad4 and are translocated into the nucleus, where they regulate transcription of target genes. Participates in a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis. Two alternatively spliced products have been described.
Protein type: Transcription, coactivator/corepressor; DNA-binding
Chromosomal Location of Human Ortholog: 13q12-q14
Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; intracellular; cytosol; nucleus
Molecular Function: protein binding; DNA binding; metal ion binding; transcription factor activity; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity
Biological Process: BMP signaling pathway; Mullerian duct regression; transcription, DNA-dependent; ureteric bud development; positive regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; cartilage development; midbrain development; response to hypoxia; positive regulation of cell differentiation; protein amino acid phosphorylation; hindbrain development
Disease: Pulmonary Hypertension, Primary, 2
Research Articles on SMAD9
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Product Notes
The Human SMAD9 smad9 (Catalog #AAA9714235) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714235 ELISA Kit recognizes Human SMAD9. It is sometimes possible for the material contained within the vial of "Mothers Against Decapentaplegic Homolog 9 (SMAD9), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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