Western Blot (WB)
(Figure 1. Western blot analysis of SRD5A1 using anti-SRD5A1 antibody (MBS1753597).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: rat liver tissue lysatesLane 2: rat kidney tissue lysatesLane 3: rat testis tissue lysatesLane 4: mouse liver tissue lysatesLane 5: mouse kidney tissue lysatesLane 6: mouse testis tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-SRD5A1 antigen affinity purified polyclonal antibody (Catalog # MBS1753597) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for SRD5A1 at approximately 29KD. The expected band size for SRD5A1 is at 29KD. )
Rabbit SRD5A1 Polyclonal Antibody | anti-SRD5A1 antibody
Anti-SRD5A1 Antibody
FC/FACS/FCM: 1-3ug/1x106 cells|Human, Rat|
Direct ELISA: 0.1-0.5ug/ml|Human|
Western Blot (WB)
(Figure 1. Western blot analysis of SRD5A1 using anti-SRD5A1 antibody (MBS1753597).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: rat liver tissue lysatesLane 2: rat kidney tissue lysatesLane 3: rat testis tissue lysatesLane 4: mouse liver tissue lysatesLane 5: mouse kidney tissue lysatesLane 6: mouse testis tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-SRD5A1 antigen affinity purified polyclonal antibody (Catalog # MBS1753597) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for SRD5A1 at approximately 29KD. The expected band size for SRD5A1 is at 29KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of SRD5A1 using anti-SRD5A1 antibody (MBS1753597).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: rat liver tissue lysatesLane 2: rat kidney tissue lysatesLane 3: rat testis tissue lysatesLane 4: mouse liver tissue lysatesLane 5: mouse kidney tissue lysatesLane 6: mouse testis tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-SRD5A1 antigen affinity purified polyclonal antibody (Catalog # MBS1753597) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for SRD5A1 at approximately 29KD. The expected band size for SRD5A1 is at 29KD. )
Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of PC-3 cells using anti-SRD5A1 antibody (MBS1753597).Overlay histogram showing PC-3 cells stained with MBS1753597 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SRD5A1 Antibody (MBS1753597, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of PC-3 cells using anti-SRD5A1 antibody (MBS1753597).Overlay histogram showing PC-3 cells stained with MBS1753597 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SRD5A1 Antibody (MBS1753597, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
2. Cantagrel, V., Lefeber, D. J., Ng, B. G., Guan, Z., Silhavy, J. L., Bielas, S. L., Lehle, L., Hombauer, H., Adamowicz, M., Swiezewska, E., De Brouwer, A. P., Blumel, P., and 13 others. SRD5A3 is required for converting polyprenol to dolichol and is mutated in a congenital glycosylation disorder. Cell 142: 203-217, 2010.
3. Ellis, J. A., Stebbing, M., Harrap, S. B. Genetic analysis of male pattern baldness and the 5-alpha-reductase genes. J. Invest. Derm. 110: 849-853, 1998.
NCBI and Uniprot Product Information
NCBI Description
Steroid 5-alpha-reductase (EC 1.3.99.5) catalyzes the conversion of testosterone into the more potent androgen, dihydrotestosterone (DHT). Also see SRD5A2 (MIM 607306).[supplied by OMIM, Mar 2008]
Uniprot Description
SRD5A1: Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5- alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology. Belongs to the steroid 5-alpha reductase family.
Protein type: Lipid Metabolism - androgen and estrogen; Membrane protein, integral; Oxidoreductase; Membrane protein, multi-pass; EC 1.3.1.22
Chromosomal Location of Human Ortholog: 5p15
Cellular Component: endoplasmic reticulum membrane; integral to membrane
Molecular Function: electron carrier activity; cholestenone 5-alpha-reductase activity; 3-oxo-5-alpha-steroid 4-dehydrogenase activity
Biological Process: steroid metabolic process; sex determination; androgen biosynthetic process; cell differentiation; sex differentiation
Research Articles on SRD5A1
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Product Notes
The SRD5A1 srd5a1 (Catalog #AAA1753597) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SRD5A1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SRD5A1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA). WB: 0.1-0.25ug/ml|Mouse, Rat| FC/FACS/FCM: 1-3ug/1x106 cells|Human, Rat| Direct ELISA: 0.1-0.5ug/ml|Human|. Researchers should empirically determine the suitability of the SRD5A1 srd5a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SRD5A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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