Testing Data
(Detection of LMW-PTP: Lysates from human CHP100 and HepG2 cells were resolved by SDS-PAGE, transferred to an Immobilon-P membrane, and immunoblotted with 1ug/ml mouse anti-LMW-PTP.)
Mouse ACP1 Monoclonal Antibody
ACP1 (Low Molecular Weight Phosphotyrosine Protein Phosphatase, LMW-PTP, LMW-PTPase, Adipocyte Acid Phosphatase, Low Molecular Weight Cytosolic Acid Phosphatase, Red Cell Acid Phosphatase 1)
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
ACP1; Monoclonal Antibody; ACP1 (Low Molecular Weight Phosphotyrosine Protein Phosphatase; LMW-PTP; LMW-PTPase; Adipocyte Acid Phosphatase; Low Molecular Weight Cytosolic Acid Phosphatase; Red Cell Acid Phosphatase 1); Anti -ACP1 (Low Molecular Weight Phosphotyrosine Protein Phosphatase; anti-ACP1 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
11C1
Specificity
Recognizes human ACP1. Species Crossreactivity: mouse, rat.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS.
Applicable Applications for anti-ACP1 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: ELISA: 0.5-1ug/ml
Western Blot: 2ug/ml
Dilution: ELISA: 0.5-1ug/ml
Western Blot: 2ug/ml
Immunogen
Purified, E.coli-derived recombinant human ACP1 (aa1-158; Accession #P24666).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
(Detection of LMW-PTP: Lysates from human CHP100 and HepG2 cells were resolved by SDS-PAGE, transferred to an Immobilon-P membrane, and immunoblotted with 1ug/ml mouse anti-LMW-PTP.)
Testing Data
(Detection of LMW-PTP: Lysates from human CHP100 and HepG2 cells were resolved by SDS-PAGE, transferred to an Immobilon-P membrane, and immunoblotted with 1ug/ml mouse anti-LMW-PTP.)
Related Product Information for anti-ACP1 antibody
Low molecular weight protein tyrosine phosphatase (LMW-PTP), also known as Acid Phosphatase 1 (ACP1), is an 18kD cytosolic phosphatase that is unrelated to other tyrosine phosphatases, such as PTP1B. Tyrosine phosphorylation of LMW-PTP increases its activity 20-fold and affects its ability to dephosphorylate targets such as the PDGF receptor. Cancer cells overexpressing LMW-PTP have a higher proliferative rate and generate tumors that are larger than untransfected controls. Colon adenocarcinomas induced by dimethylhydrazine also have elevated levels of LMW-PTP, suggesting that this phosphatase may be a marker for oncogenic transformation.
Product Categories/Family for anti-ACP1 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The ACP1 (Catalog #AAA609574) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ACP1 (Low Molecular Weight Phosphotyrosine Protein Phosphatase, LMW-PTP, LMW-PTPase, Adipocyte Acid Phosphatase, Low Molecular Weight Cytosolic Acid Phosphatase, Red Cell Acid Phosphatase 1) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ACP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: ELISA: 0.5-1ug/ml Western Blot: 2ug/ml. Researchers should empirically determine the suitability of the ACP1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACP1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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