Rabbit anti-Human SRC8 Polyclonal Antibody | anti-SRC8 antibody

SRC8, NT (CTTN, EMS1, Src substrate cortactin, Amplaxin, Oncogene EMS1) (FITC)

Cat. Num. AAA6351084

• Gene Names: CTTN; EMS1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SRC8; Polyclonal Antibody; NT (CTTN; EMS1; Src substrate cortactin; Amplaxin; Oncogene EMS1) (FITC); CTTN; Oncogene EMS1; anti-SRC8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-SRC8 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SRC8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16~46 amino acids from the N-terminal region of human SRC8.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of SRC8 Antibody (N-term) in HL-60 cell line lysates (35ug/lane). SRC8 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human breast carcinoma with SRC8 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-SRC8 antibody

Antibodies; Proteins

References

Xu, X.Z., et.al, Mod. Pathol. 23 (2), 187-196 (2010). Saitoh, Y., et.al, Int. J. Oncol. 35 (6), 1277-1288 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 296080746
• NCBI GeneID: 2017
• NCBI Accession #: NP_001171669.1
• NCBI GenBank Nucleotide #: NM_001184740.1
• UniProt Accession #: Q14247; Q8N707; Q96H99; AAB26248
• Molecular Weight: 61,586 Da
• NCBI Official Full Name: src substrate cortactin isoform c
• NCBI Official Synonym Full Names: cortactin
• NCBI Official Symbol: CTTN
• NCBI Official Synonym Symbols: EMS1
• NCBI Protein Information: src substrate cortactin; amplaxin; 1110020L01Rik; oncogene EMS1; ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 src substrate)
• UniProt Protein Name: Src substrate cortactin
• UniProt Gene Name: CTTN
• UniProt Synonym Gene Names: EMS1
• UniProt Entry Name: SRC8_HUMAN

NCBI Description

This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq, May 2010]

Uniprot Description

Cortactin: a cytoskeletal protein that that is involved in coordinating actin reorganization during cell movement. Localizes at the leading edge of lamellipodia during cell migration. Its amino-terminal acidic domain associates with the Arp2/3 and WASP complex at F-actin branches. The central region of the protein contains six repeats of 37 amino acids that are important in F-actin binding and cross-linking. The carboxy-terminus contains a proline-rich region and an SH3 domain that can interact with numerous scaffolding proteins, such as CortBP1 and Shank3. Cortactin may play a role in the organization of transmembrane receptors at postsynaptic densities (PSD) and tight junctions by linking scaffolding proteins to the actin network. It is degraded in a caspase-dependent manner during apoptosis. It is overexpressed in numerous cancers with poor patient prognosis. It may contribute to tumor cell invasion and metastasis. Two splice variant isoforms have been identified.

Protein type: Motility/polarity/chemotaxis; Actin-binding; Cytoskeletal

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: cortical cytoskeleton; voltage-gated potassium channel complex; focal adhesion; dendritic spine; actin filament; coated pit; cell cortex; ruffle; growth cone; cytoskeleton; lamellipodium; cytoplasm; podosome

Molecular Function: protein binding

Biological Process: focal adhesion formation; intracellular protein transport; receptor-mediated endocytosis; positive regulation of actin filament polymerization; actin filament polymerization; actin cytoskeleton reorganization; cell motility involved in cell locomotion; regulation of axon extension; substrate-bound cell migration, cell extension; neurite morphogenesis