Immunohistochemistry (IHC)
(Immunochemical staining of human SPTLC2 in human hepatoma with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Rabbit anti-Human SPTLC2/Serine Palmitoyltransferase Polyclonal Antibody | anti-SPTLC2 antibody
Anti-SPTLC2/Serine Palmitoyltransferase Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
SPTLC2/Serine Palmitoyltransferase; Polyclonal Antibody; Anti-SPTLC2/Serine Palmitoyltransferase Antibody; Rabbit Polyclonal; SPTLC2/Serine Palmitoyltransferase Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-Serine Palmitoyltransferase Antibody; Anti-SPTLC2 Antibody; anti-SPTLC2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human SPTLC2/Serine Palmitoyltransferase
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-SPTLC2 antibody
Immunohistochemistry-Paraffin (IHC-P)
Application Notes
IHC-P: 1:50-1:200
Immunogen
E Coli-derived Human SPTLC2/Serine Palmitoyltransferase fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human SPTLC2/Serine Palmitoyltransferase fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of human SPTLC2 in human hepatoma with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human SPTLC2 in human hepatoma with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-SPTLC2 antibody
Serine palmitoyltransferase (SPT) is the rate-limiting enzyme for sphingolipid biosynthesis. SPT has two major subunits, SPTLC1 and SPTLC2. In the human genome, SPTLC1 comprises 15 exons spanning ~85 kbp in the chromosome 9q21-q22 region, and SPTLC2 comprises 12 exons spanning ~110 kbp in the chromosome 14q24.3-q31 region. SPTLC1 and SPTLC2 encode subunits 1 and 2 of serine palmitoyltransferase (SPT), an enzyme that catalyzes the biosynthesis of serine and palmitoyl CoA. Upregulation of SPT is suggested to play a role in apoptosis. Gain of function mutations in the SPTLC1 and SPTLC2 genes lead to the formation of atypical deoxysphingoid bases that cannot be converted to complex sphingolipids or degraded, resulting in their intracellular accumulation with pronounced neurotoxic effects on neurite formation and neurofilament structure.
References
Suriyanarayanan S, et al. (2016) The variant p.(arg183trp) in sptlc2 causes late-onset hereditary sensory neuropathy. Neuromolecular Med 18 (1): 81-90.Li Z, et al. (2018) Sphingolipid de novo biosynthesis is essential for intestine cell survival and barrier function. Cell Death Dis 9 (2): 173.Houlden H, et al. (2004) Hereditary sensory neuropathies. Curr Opin Neurol 17 (5): 569-577.
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Product Notes
The SPTLC2 (Catalog #AAA8109160) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SPTLC2/Serine Palmitoyltransferase Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SPTLC2/Serine Palmitoyltransferase can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P). IHC-P: 1:50-1:200. Researchers should empirically determine the suitability of the SPTLC2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPTLC2/Serine Palmitoyltransferase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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