Rabbit anti-Human, Mouse SPT16 (Intermediate Domain) Polyclonal Antibody | anti-SPT16 antibody
SPT16 (Intermediate Domain)
NCBI and Uniprot Product Information
Uniprot Description
Function: Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Binds specifically to double-stranded DNA. Also acts as a transcriptional coactivator for p63/TP63.
Subunit structure: Component of the FACT complex, a stable heterodimer of SSRP1 and SUPT16H. Also component of a CK2-SPT16-SSRP1 complex which forms following UV irradiation, composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B. Binds to histone H3-H4 tetramers, but not to intact nucleosomes. Interacts with isoform gamma of p63/TP63. Interacts with FYTTD1/UIF, SRF and NEK9
By similarity.
Subcellular location: Nucleus. Nucleus › nucleolus
By similarity. Chromosome. Note: Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci
By similarity.
Post-translational modification: Phosphorylated by CK2 following UV but not gamma irradiation. Phosphorylation inhibits its DNA-binding activity
By similarity.Ubiquitinated. Polyubiquitinated following caspase cleavage resulting in degradation of the N-terminal ubiquitinated part of the cleaved protein
By similarity.Sumoylated
By similarity.
Disruption phenotype: Embryos die soon after implantation while preimplantation blastocysts are defective for cell outgrowth. Ref.4
Sequence similarities: Belongs to the SSRP1 family.Contains 1 HMG box DNA-binding domain.
Sequence caution: The sequence AAH42502.1 differs from that shown. Reason: Frameshift at position 697.
Research Articles on SPT16
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Product Notes
The SPT16 ssrp1 (Catalog #AAA395740) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SPT16 (Intermediate Domain) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SPT16 (Intermediate Domain) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the SPT16 ssrp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPT16 (Intermediate Domain), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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