Rabbit anti-Human SPT1 Polyclonal Antibody | anti-SPT1 antibody

SPT1 Antibody

Cat. Num. AAA9406348

• Gene Names: Sptlc1; Lcb1; C77762; AW552086; E030036H05
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity chromatography purified via peptide column
• Synonyms: SPT1; Polyclonal Antibody; SPT1 Antibody; Serine palmitoyltransferase long chain base subunit 1; SPTLC1; HSAN; HSN; LBC1; LCB1; SPTI; anti-SPT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: At least two isoforms of SPT1 are known to exist; this antibody will detect only isoform a. SPT1 antibody is predicted to not cross-react with SPT2.
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 473

• Applicable Applications for anti-SPT1 antibody: ELISA (EIA), Western Blot (WB)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 17 amino acid peptide near the carboxy terminus of human SPT1.
• Target Name: SPT1
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of SPT1 in human lung tissue lysate with SPT1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.)

Related Product Information for anti-SPT1 antibody

Serine palmitoyltransferase, which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate as a cofactor. SPT1 is the long chain base subunit 1 of mammalian serine palmitoyltransferase. SPT1 is not catalytically active but is necessary for the stabilization of the SPT2 subunit and anchoring the holoenzyme to the cytosolic face of the endoplasmic reticulum. Missense mutations in this gene have been identified in patients with hereditary sensory neuropathy type 1 (HSAN1). These mutations induce a shift in the substrate specificity of the holoenzyme, leading to the formation and accumulation of two neurotoxic sphingolipids.

Product Categories/Family for anti-SPT1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 29244577
• NCBI GeneID: 268656
• NCBI Accession #: NP_033295.2
• NCBI GenBank Nucleotide #: NM_009269.2
• Molecular Weight: 52,535 Da
• NCBI Official Full Name: serine palmitoyltransferase 1
• NCBI Official Synonym Full Names: serine palmitoyltransferase, long chain base subunit 1
• NCBI Official Symbol: Sptlc1
• NCBI Official Synonym Symbols: Lcb1; C77762; AW552086; E030036H05
• NCBI Protein Information: serine palmitoyltransferase 1
• UniProt Protein Name: Serine palmitoyltransferase 1
• UniProt Gene Name: Sptlc1
• UniProt Synonym Gene Names: Lcb1; LCB 1; SPT 1; SPT1
• UniProt Entry Name: SPTC1_MOUSE

Uniprot Description

SPTLC1: Serine palmitoyltransferase (SPT). The heterodimer formed with SPTLC2 or SPTLC3 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. The SPTLC1- SPTLC2-SPTSSB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference. Defects in SPTLC1 are the cause of hereditary sensory and autonomic neuropathy type 1A (HSAN1A). The hereditary sensory and autonomic neuropathies are a genetically and clinically heterogeneous group of disorders characterized by degeneration of dorsal root and autonomic ganglion cells, and by sensory and/or autonomic abnormalities. HSAN1A is an autosomal dominant axonal neuropathy with onset in the second or third decades. Initial symptoms are loss of pain, touch, heat, and cold sensation over the feet, followed by distal muscle wasting and weakness. Loss of pain sensation leads to chronic skin ulcers and distal amputations. Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.3.1.50; Lipid Metabolism - sphingolipid; Transferase; Membrane protein, integral

Cellular Component: membrane; endoplasmic reticulum; integral to membrane

Molecular Function: transferase activity; serine C-palmitoyltransferase activity; protein binding; transferase activity, transferring acyl groups; catalytic activity; pyridoxal phosphate binding

Biological Process: sphingolipid metabolic process; sphingosine biosynthetic process; ceramide biosynthetic process; biosynthetic process; sphinganine biosynthetic process; lipid metabolic process; sphingomyelin biosynthetic process