<
Western Blot (WB)
(Western blot analysis of extracts from mouse brain, using TACC1 Antibody. Lane 1 was treated with the blocking peptide.)
Rabbit anti-Human, Mouse TACC1 Polyclonal Antibody | anti-TACC1 antibody
TACC1 Antibody
Gene Names
TACC1; Ga55
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
TACC1; Polyclonal Antibody; TACC1 Antibody; DKFZp686K18126; Ga 55; Ga55; Gastric cancer antigen Ga 55; Gastric cancer antigen Ga55; KIAA1103; TACC 1; Tacc1; TACC1_HUMAN; Taxin 1; Taxin-1; Taxin1; Transforming acidic coiled coil containing protein 1; Transforming acidic coiled-coil-containing protein 1; anti-TACC1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
TACC1 antibody detects endogenous levels of TACC1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
395
Applicable Applications for anti-TACC1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human TACC1
Subcellular Location
Cytoplasm. Nucleus. Cytoplasm > Cytoskeleton > Centrosome. Nucleus during interphase. Weakly concentrated at centrosomes during mitosis and colocalizes with AURKC at the midbody during cytokinesis.
Tissue Specificity
Isoform 1, isoform 3 and isoform 5 are ubiquitous. Isoform 2 is strongly expressed in the brain, weakly detectable in lung and colon, and overexpressed in gastric cancer. Isoform 4 is not detected in normal tissues, but strong expression was found in gastric cancer tissues. Down-regulated in a subset of cases of breast cancer.
Predicted Cross Reactivity
Bovine, Horse, Sheep, Rabbit, Dog
Similarity
Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from mouse brain, using TACC1 Antibody. Lane 1 was treated with the blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from mouse brain, using TACC1 Antibody. Lane 1 was treated with the blocking peptide.)
Immunofluorescene (IF)
(MBS9600355 staining K562 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescene (IF)
(MBS9600355 staining K562 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9600355 staining K562 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunofluorescence (IF)
(MBS9600355 staining K562 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Western Blot (WB)
(Western blot analysis on K562 cell lysate using TACC1 Antibody, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis on K562 cell lysate using TACC1 Antibody, The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-TACC1 antibody
Function: Likely involved in the processes that promote cell division prior to the formation of differentiated tissues.
Subunit Structure: Interacts with KIAA0097/CH-TOG and with the oncogenic transcription factor YEATS4. Interacts with AURKA, AURKB and AURKC. Interacts with LSM7, TDRD7 and SNRPG. Interacts with GCN5L2 and PCAF.
Post-translational Modifications: Isoform 1 is heavily phosphorylated; isoform 6 is not.
Similarity: Belongs to the TACC family.
Subunit Structure: Interacts with KIAA0097/CH-TOG and with the oncogenic transcription factor YEATS4. Interacts with AURKA, AURKB and AURKC. Interacts with LSM7, TDRD7 and SNRPG. Interacts with GCN5L2 and PCAF.
Post-translational Modifications: Isoform 1 is heavily phosphorylated; isoform 6 is not.
Similarity: Belongs to the TACC family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 87 kDa
Predicted: 88 kDa
Predicted: 88 kDa
NCBI Official Full Name
transforming acidic coiled-coil-containing protein 1 isoform 2
NCBI Official Synonym Full Names
transforming acidic coiled-coil containing protein 1
NCBI Official Symbol
TACC1
NCBI Official Synonym Symbols
Ga55
NCBI Protein Information
transforming acidic coiled-coil-containing protein 1
UniProt Protein Name
Transforming acidic coiled-coil-containing protein 1
Protein Family
UniProt Gene Name
TACC1
UniProt Synonym Gene Names
KIAA1103
NCBI Description
This locus may represent a breast cancer candidate gene. It is located close to FGFR1 on a region of chromosome 8 that is amplified in some breast cancers. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2017]
Uniprot Description
Likely involved in the processes that promote cell division prior to the formation of differentiated tissues.
Research Articles on TACC1
Similar Products
Product Notes
The TACC1 tacc1 (Catalog #AAA9600355) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TACC1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TACC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:2000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the TACC1 tacc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TACC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
