Rabbit anti-Human, Mouse SPPL2A Polyclonal Antibody | anti-SPPL2A antibody

SPPL2A Antibody

Cat. Num. AAA9630886

• Gene Names: SPPL2A; IMP3; PSL2
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: SPPL2A; Polyclonal Antibody; SPPL2A Antibody; EC 3.4.99; IMP3; Intramembrane protease 3; Presenilin like protein 2; PSL2; Signal peptide peptidase like 2A; SPP like 2A protein; anti-SPPL2A antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: SPPL2A Antibody detects endogenous levels of SPPL2A.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-SPPL2A antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human SPPL2A.
• Conjugation: Unconjugated
• Similarity: The PAL motif is required for normal active site conformation. The catalytic domains embedded in the membrane are in the opposite orientation to that of the presenilin protein family; therefore, it is predicted to cleave type II-oriented substrate peptides like the prototypic protease SPP. The C-terminal tail is necessary for lysosomal transport. Belongs to the peptidase A22B family.
• Post Translational Modifications: Glycosylated.
• Subunit Structure: Interacts with ITM2B.
• Tissue Specificity: Ubiquitous.
• Function: Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane signal peptides in the hydrophobic plane of the membrane. Functions in FASLG, ITM2B and TNF processing. Catalyzes the intramembrane cleavage of the anchored fragment of shed TNF-alpha (TNF), which promotes the release of the intracellular domain (ICD) for signaling to the nucleus. Also responsible for the intramembrane cleavage of Fas antigen ligand FASLG, which promotes the release of the intracellular FasL domain (FasL ICD). May play a role in the regulation of innate and adaptive immunity. Catalyzes the intramembrane cleavage of the simian foamy virus envelope glycoprotein gp130 independently of prior ectodomain shedding by furin or furin-like proprotein convertase (PC)-mediated cleavage proteolysis.
• Note: Colocalizes with palmitoylated and myristoylated proteins at the plasma membrane.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-SPPL2A antibody

Late endosome membrane; Multi-pass membrane protein; Lysosome membrane; Membrane; Lumenal side; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 21314755
• NCBI GeneID: 84888
• NCBI Accession #: NP_116191.2
• NCBI GenBank Nucleotide #: NM_032802.3
• UniProt Accession #: Q8TCT8; Q8TAW1; Q96SZ8; B2RDS0
• Molecular Weight: 58,143 Da
• NCBI Official Full Name: signal peptide peptidase-like 2A
• NCBI Official Symbol: SPPL2A
• NCBI Official Synonym Symbols: IMP3; PSL2
• NCBI Protein Information: signal peptide peptidase-like 2A; IMP-3; SPP-like 2A; OTTHUMP00000162418; intramembrane protease 3; presenilin-like protein 2; intramembrane cleaving protease
• UniProt Protein Name: Signal peptide peptidase-like 2A
• Protein Family: Signal peptide peptidase
• UniProt Gene Name: SPPL2A
• UniProt Synonym Gene Names: IMP3; PSL2
• UniProt Entry Name: PSL2_HUMAN

NCBI Description

This gene is a member of the signal peptide peptidase-like protease (SPPL) family and encodes an endosomal membrane protein with a protease associated (PA) domain. This protein plays a role in innate and adaptive immunity. A pseudogene of this gene also lies on chromosome 15. [provided by RefSeq]

Uniprot Description

SPPL2A: Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane signal peptides in the hydrophobic plane of the membrane. Functions in FASLG, ITM2B and TNF processing. Catalyzes the intramembrane cleavage of the anchored fragment of shed TNF-alpha (TNF), which promotes the release of the intracellular domain (ICD) for signaling to the nucleus. Also responsible for the intramembrane cleavage of Fas antigen ligand FASLG, which promotes the release of the intracellular FasL domain (FasL ICD). May play a role in the regulation of innate and adaptive immunity. Belongs to the peptidase A22B family.

Protein type: EC 3.4.23.-; Membrane protein, multi-pass; Cancer Testis Antigen (CTA); Protease; Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q21.2

Cellular Component: lysosomal membrane; late endosome membrane; plasma membrane

Molecular Function: protein binding; protein homodimerization activity; aspartic-type endopeptidase activity

Biological Process: regulation of immune response; membrane protein intracellular domain proteolysis; membrane protein ectodomain proteolysis