Rabbit anti-Human PRMT7 Polyclonal Antibody | anti-PRMT7 antibody

PRMT7, CT (Protein Arginine N-methyltransferase 7, PRMT-7, Histone-arginine N-methyltransferase PRMT7, [Myelin Basic Protein]-arginine N-methyltransferase PRMT7, KIAA1933)

Cat. Num. AAA630285

• Gene Names: PRMT7; FLJ10640; KIAA1933
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified; Purified by ammonium sulfate precipitation.
• Synonyms: PRMT7; Polyclonal Antibody; CT (Protein Arginine N-methyltransferase 7; PRMT-7; Histone-arginine N-methyltransferase PRMT7; [Myelin Basic Protein]-arginine N-methyltransferase PRMT7; KIAA1933); Anti -PRMT7; anti-PRMT7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PRMT7.
• Purity/Purification: Purified; Purified by ammonium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-PRMT7 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide in the C-term region of human PRMT7 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PRMT7 (arrow) using rabbit polyclonal PRMT7 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRMT7 gene (Lane 2).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-PRMT7 antibody

Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins, IL enhancer binding factor, nuclear factors, cell cycle proteins, signal transduction proteins, apoptosis proteins, and viral proteins. The mammalian PRMT family currently consists of 7 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all 7 PRMT members have been developed.

Product Categories/Family for anti-PRMT7 antibody

Antibodies; Abs to Enzymes; Methyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 296317343
• NCBI GeneID: 54496
• NCBI Accession #: NP_001171753.1
• NCBI GenBank Nucleotide #: NM_001184824.1
• UniProt Accession #: Q9NVM4; Q96PV5; Q9H9L0; B3KPR0; B3KUG9; B4E379
• Molecular Weight: 78,459 Da
• NCBI Official Full Name: protein arginine N-methyltransferase 7 isoform 2
• NCBI Official Synonym Full Names: protein arginine methyltransferase 7
• NCBI Official Symbol: PRMT7
• NCBI Official Synonym Symbols: FLJ10640; KIAA1933
• NCBI Protein Information: protein arginine N-methyltransferase 7; OTTHUMP00000174863; histone-arginine N-methyltransferase PRMT7; myelin basic protein-arginine N-methyltransferase
• UniProt Protein Name: Protein arginine N-methyltransferase 7
• UniProt Gene Name: PRMT7
• UniProt Synonym Gene Names: KIAA1933
• UniProt Entry Name: ANM7_HUMAN

NCBI Description

Arginine methylation is an apparently irreversible protein modification catalyzed by arginine methyltransferases, such as PMT7, using S-adenosylmethionine (AdoMet) as the methyl donor. Arginine methylation is implicated in signal transduction, RNA transport, and RNA splicing (Miranda et al., 2004 [PubMed 15044439]).[supplied by OMIM]

Uniprot Description

PRMT7: Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Specifically mediates the symmetric dimethylation of histone H4 'Arg-3' to form H4R3me2s. Plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3me2s, possibly leading to recruit DNA methyltransferases at these sites. May also play a role in embryonic stem cell (ESC) pluripotency. Also able to mediate the arginine methylation of histone H2A and myelin basic protein (MBP) in vitro; the relevance of such results is however unclear in vivo. Belongs to the protein arginine N-methyltransferase family. PRMT7 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase; EC 2.1.1.125; EC 2.1.1.126; Methyltransferase, protein arginine; RNA processing

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: nucleoplasm; nucleolus; plasma membrane; cytosol; nucleus

Molecular Function: protein-arginine omega-N monomethyltransferase activity; ribonucleoprotein binding; protein-arginine omega-N asymmetric methyltransferase activity; [myelin basic protein]-arginine N-methyltransferase activity; histone binding; protein-arginine omega-N symmetric methyltransferase activity; S-adenosylmethionine-dependent methyltransferase activity; histone-arginine N-methyltransferase activity

Biological Process: histone methylation; peptidyl-arginine methylation, to symmetrical-dimethyl arginine; spliceosomal snRNP biogenesis; peptidyl-arginine methylation; establishment and/or maintenance of chromatin architecture; genetic imprinting; regulation of transcription, DNA-dependent; transcription, DNA-dependent; DNA methylation during gametogenesis; regulation of protein binding; peptidyl-arginine methylation, to asymmetrical-dimethyl arginine; cell differentiation