Western Blot (WB)
(Western blot analysis of sPLA2-IID expression in Hela (A), THP1 (B) whole cell lysates.)
Rabbit anti-Human sPLA2-IID Polyclonal Antibody | anti-PLA2G2D antibody
Anti-sPLA2-IID Antibody
Western Blot (WB)
(Western blot analysis of sPLA2-IID expression in Hela (A), THP1 (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of sPLA2-IID expression in Hela (A), THP1 (B) whole cell lysates.)
Immunofluorescence (IF)
(Immunofluorescent analysis of sPLA2-IID staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of sPLA2-IID staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a secreted member of the phospholipase A2 family, and is found in a cluster of related family members on chromosome 1. Phospholipase A2 family members hydrolyze the sn-2 fatty acid ester bond of glycerophospholipids to produce lysophospholipids and free fatty acid. This gene may be involved in inflammation and immune response, and in weight loss associated with chronic obstructive pulmonary disease. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2012]
Uniprot Description
PLA2G2D: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. L-alpha-1-palmitoyl-2- linoleoyl phosphatidylethanolamine is more efficiently hydrolyzed than the other phospholipids examined. Belongs to the phospholipase A2 family.
Protein type: Lipid Metabolism - linoleic acid; Lipid Metabolism - ether lipid; Lipid Metabolism - glycerophospholipid; Secreted, signal peptide; Secreted; Lipid Metabolism - arachidonic acid; Lipid Metabolism - alpha-linolenic acid; EC 3.1.1.4; Phospholipase
Chromosomal Location of Human Ortholog: 1p36.12
Cellular Component: extracellular region
Molecular Function: calcium ion binding; heparan sulfate proteoglycan binding; heparin binding; phospholipase A2 activity
Biological Process: CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation; inflammatory response; lipid catabolic process; negative regulation of T cell proliferation; phosphatidic acid biosynthetic process; phospholipid metabolic process
Research Articles on PLA2G2D
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Product Notes
The PLA2G2D pla2g2d (Catalog #AAA8245205) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-sPLA2-IID Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's sPLA2-IID can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1/500 - 1/2000; IF/ICC: 1/10 - 1/100. Researchers should empirically determine the suitability of the PLA2G2D pla2g2d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "sPLA2-IID, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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