Typical Testing Data/Standard Curve (for reference only)
Human Phospholipase A2, Group IID (PLA2G2D) RTU ELISA Kit | PLA2G2D rtu elisa kit
Human Phospholipase A2, Group IID (PLA2G2D) ELISA Kit
Gene Names
PLA2G2D; SPLASH; sPLA2S; PLA2IID; sPLA2-IID
Reactivity
Human
Synonyms
Phospholipase A2; Group IID (PLA2G2D); Human Phospholipase A2; Group IID (PLA2G2D) ELISA Kit; PLA2G2D; sPLA2S; s-PLA2; sPLA2; SPLASH; Secreted Phospholipase A2; Phosphatidylcholine 2-acylhydrolase 2D; Secretory-type PLA; stroma-associated homolog; PLA2G2D rtu elisa kit
Reactivity
Human
Sequence Length
62
Assay Type
Sandwich
Samples
Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
Detection Range
0.937-60ng/mL
Sensitivity
0.32ng/mL
RTU ELISA Kit's Advantage
The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
Preparation and Storage
For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PLA2G2D rtu elisa kit
Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PLA2G2D. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PLA2G2D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PLA2G2D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PLA2G2D in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16,546 Da
NCBI Official Full Name
group IID secretory phospholipase A2 isoform 2
NCBI Official Synonym Full Names
phospholipase A2 group IID
NCBI Official Symbol
PLA2G2D
NCBI Official Synonym Symbols
SPLASH; sPLA2S; PLA2IID; sPLA2-IID
NCBI Protein Information
group IID secretory phospholipase A2
UniProt Protein Name
Group IID secretory phospholipase A2
Protein Family
UniProt Gene Name
PLA2G2D
UniProt Synonym Gene Names
SPLASH; GIID sPLA2; sPLA2-IID
NCBI Description
This gene encodes a secreted member of the phospholipase A2 family, and is found in a cluster of related family members on chromosome 1. Phospholipase A2 family members hydrolyze the sn-2 fatty acid ester bond of glycerophospholipids to produce lysophospholipids and free fatty acid. This gene may be involved in inflammation and immune response, and in weight loss associated with chronic obstructive pulmonary disease. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2012]
Uniprot Description
PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. L-alpha-1-palmitoyl-2-linoleoyl phosphatidylethanolamine is more efficiently hydrolyzed than the other phospholipids examined.
Research Articles on PLA2G2D
Similar Products
Product Notes
The Human PLA2G2D pla2g2d (Catalog #AAA4501896) is a RTU ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA4501896 RTU ELISA Kit recognizes Human PLA2G2D. It is sometimes possible for the material contained within the vial of "Phospholipase A2, Group IID (PLA2G2D), RTU ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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