Cyclic Guanosine Monophosphate (cGMP) ELISA Kit | cGMP elisa kit

General species Cyclic Guanosine Monophosphate (cGMP)(Competitive EIA) ELISA Kit

Cat. Num. AAA2125218

• Reactivity: General species
• Applications: ELISA
• Synonyms: Cyclic Guanosine Monophosphate (cGMP); General species Cyclic Guanosine Monophosphate (cGMP)(Competitive EIA) ELISA Kit; Mini Samples ELISA Kit for Cyclic Guanosine Monophosphate (cGMP); cGMP/Cyclic Guanosine Monophosphate; c-GMP; 3'; 5'-Cyclic GMP; Cyclic 3'; 5'-GMP; Guanosine 3'; 5'-Cyclic Phosphate; cGMP elisa kit

• Reactivity: General species
• Specificity: Reactive with General species Cyclic Guanosine Monophosphate (cGMP); This assay has high sensitivity and excellent specificity for detection of Mini Samples Cyclic Guanosine Monophosphate (cGMP). No significant cross-reactivity or interference between Mini Samples Cyclic Guanosine Monophosphate (cGMP) and analogues was observed.

• Applicable Applications for cGMP elisa kit: ELISA (EIA)
• Application Notes: Enzyme-linked immunosorbent assay for Antigen Detection.
• Assay Type: Competitive ELISA-Mini Samples
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 123.5-10,000pg/mL
• Sensitivity: 49.6pg/mL
• Detection Method: Competitive Inhibition
• Assay Length: 3 hours
• Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Cyclic Guanosine Monophosphate (cGMP) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Cyclic Guanosine Monophosphate (cGMP) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100.; Intra-Assay: CV<10%.; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein C1 (APOC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein C1 (APOC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein C1 (APOC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Apolipoprotein C1 (APOC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Assay Procedure Summary: 1. Prepare all reagents, samples and standards; 2. Add 25uL standard or sample to each well. And then add 25?L prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37 degree C; 3. Aspirate and wash 3 times; 4. Add 50uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C; 5. Aspirate and wash 5 times; 6. Add 50uL Substrate Solution. Incubate 10-20 minutes at 37 degree C; 7. Add 25uL Stop Solution. Read at 450 nm immediately.
• Preparation and Storage: Store at 4 degree C for 1 month; -20 degree C for long-term (within shelf life); The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Product Categories/Family for cGMP elisa kit

Metabolic pathway