Rabbit anti-Human SPINT2 Polyclonal Antibody | anti-SPINT2 antibody

SPINT2, CT (SPINT2, HAI2, KOP, Kunitz-type protease inhibitor 2, Hepatocyte growth factor activator inhibitor type 2, Placental bikunin) (Azide free) (HRP)

Cat. Num. AAA6350600

• Gene Names: SPINT2; PB; Kop; HAI2; DIAR3; HAI-2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SPINT2; Polyclonal Antibody; CT (SPINT2; HAI2; KOP; Kunitz-type protease inhibitor 2; Hepatocyte growth factor activator inhibitor type 2; Placental bikunin) (Azide free) (HRP); Placental bikunin; anti-SPINT2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-SPINT2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SPINT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 222-251 amino acids from the C-terminal region of human SPINT2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SPINT2 Antibody (C-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the SPINT2 antibody detected the SPINT2 protein (arrow).)

Related Product Information for anti-SPINT2 antibody

This gene encodes a transmembrane protein with two extracellular Kunitz domains that inhibits a variety of serine proteases. The protein inhibits HGF activator which prevents the formation of active hepatocyte growth factor. This gene is a putative tumor suppressor, and mutations in this gene result in congenital sodium diarrhea. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Product Categories/Family for anti-SPINT2 antibody

Antibodies; Protease Inhibitors

References

Dong, W., et al. Int. J. Cancer 127(7):1526-1534(2010). Bergum, C., et al. Prostate 70(13):1422-1428(2010). Sivagnanam, M., et al. Clin. Dysmorphol. 19 (1), 48 (2010): Yamauchi, M., et al. Biol. Chem. 383(12):1953-1957(2002). Itoh, H., et al. Eur. J. Biochem. 267(11):3351-3359(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 10863909
• NCBI GeneID: 10653
• NCBI Accession #: NP_066925.1
• NCBI GenBank Nucleotide #: NM_021102.3
• UniProt Accession #: O43291; O00271; O14895; Q5TZQ3; Q969E0; A8K667; B4DLU1
• Molecular Weight: 21,828 Da
• NCBI Official Full Name: kunitz-type protease inhibitor 2 isoform a
• NCBI Official Synonym Full Names: serine peptidase inhibitor, Kunitz type, 2
• NCBI Official Symbol: SPINT2
• NCBI Official Synonym Symbols: PB; Kop; HAI2; DIAR3; HAI-2
• NCBI Protein Information: kunitz-type protease inhibitor 2; placental bikunin; serine protease inhibitor, Kunitz type, 2; hepatocyte growth factor activator inhibitor type 2
• UniProt Protein Name: Kunitz-type protease inhibitor 2
• Protein Family: Kunitz-type protease inhibitor
• UniProt Gene Name: SPINT2
• UniProt Synonym Gene Names: HAI2; KOP; HAI-2
• UniProt Entry Name: SPIT2_HUMAN

NCBI Description

This gene encodes a transmembrane protein with two extracellular Kunitz domains that inhibits a variety of serine proteases. The protein inhibits HGF activator which prevents the formation of active hepatocyte growth factor. This gene is a putative tumor suppressor, and mutations in this gene result in congenital sodium diarrhea. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

SPINT2: Inhibitor of HGF activator. Also inhibits plasmin, plasma and tissue kallikrein, and factor XIa. Defects in SPINT2 are the cause of diarrhea type 3 (DIAR3); also known as congenital sodium diarrhea (CSD). DIAR3 is a rare, inherited diarrhea of infancy. A diagnosis of DIAR3 is made on the findings of a life-threatening secretory diarrhea, severe metabolic acidosis, and hyponatremia secondary to extraordinarily high fecal losses of sodium, with low or normal excretion of urinary sodium, in the absence of infectious, autoimmune, and endocrine causes. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Inhibitor; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: cytoplasm; extracellular region

Molecular Function: endopeptidase inhibitor activity; serine-type endopeptidase inhibitor activity

Biological Process: cell motility; negative regulation of cell-cell adhesion

Disease: Diarrhea 3, Secretory Sodium, Congenital, With Or Without Other Congenital Anomalies