Human TERF2IP ELISA Kit | TERF2IP elisa kit

Human TERF2IP (Telomeric Repeat Binding Factor 2 Interacting Protein) ELISA Kit

Cat. Num. AAA2503845

• Gene Names: TERF2IP; RAP1; DRIP5
• Reactivity: Human
• Synonyms: TERF2IP; Human TERF2IP (Telomeric Repeat Binding Factor 2 Interacting Protein) ELISA Kit; TERF2IP elisa kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant Human TERF2IP. No significant cross-reactivity or interference between Human TERF2IP and analogues was observed.
• Sequence Length: 399

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 1.563-100ng/mL
• Sensitivity: Min: 0.938ng/mL; Max: 100ng/mL
• Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human TERF2IP concentrations in serum, plasma and other biological fluids.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TERF2IP elisa kit

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TERF2IP. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TERF2IP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TERF2IP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TERF2IP. You can calculate the concentration of TERF2IP in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 52627149
• NCBI GeneID: 54386
• NCBI Accession #: NP_061848.2
• NCBI GenBank Nucleotide #: NM_018975.3
• UniProt Accession #: Q9NYB0; Q4W4Y2; Q8WYZ3; Q9NWR2; B4DQN4
• Molecular Weight: 44,260 Da
• NCBI Official Full Name: telomeric repeat-binding factor 2-interacting protein 1
• NCBI Official Synonym Full Names: telomeric repeat binding factor 2, interacting protein
• NCBI Official Symbol: TERF2IP
• NCBI Official Synonym Symbols: RAP1; DRIP5
• NCBI Protein Information: telomeric repeat-binding factor 2-interacting protein 1; RAP1 homolog; TERF2-interacting telomeric protein 1; TRF2-interacting telomeric RAP1 protein; TRF2-interacting telomeric protein 1; dopamine receptor interacting protein 5; dopamine receptor-interacting protein 5; hRap1; repressor/activator protein 1 homolog
• UniProt Protein Name: Telomeric repeat-binding factor 2-interacting protein 1
• Protein Family: Telomeric repeat-binding factor 2-interacting protein
• UniProt Gene Name: TERF2IP
• UniProt Synonym Gene Names: DRIP5; RAP1; TERF2-interacting telomeric protein 1; TRF2-interacting telomeric protein 1; RAP1 homolog; hRap1
• UniProt Entry Name: TE2IP_HUMAN

NCBI Description

The gene encodes a protein that is part of a complex involved in telomere length regulation. Pseudogenes are present on chromosomes 5 and 22. [provided by RefSeq, Apr 2010]

Uniprot Description

TERF2IP: Acts both as a regulator of telomere function and as a transcription regulator. Involved in the regulation of telomere length and protection as a component of the shelterin complex (telosome). In contrast to other components of the shelterin complex, it is dispensible for telomere capping and does not participate in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair. Instead, it is required to negatively regulate telomere recombination and is essential for repressing homology-directed repair (HDR), which can affect telomere length. Does not bind DNA directly: recruited to telomeric double-stranded 5'-TTAGGG-3' repeats via its interaction with TERF2. Independently of its function in telomeres, also acts as a transcription regulator: recruited to extratelomeric 5'- TTAGGG-3' sites via its association with TERF2 or other factors, and regulates gene expression. When cytoplasmic, associates with the I-kappa-B-kinase (IKK) complex and acts as a regulator of the NF-kappa-B signaling by promoting IKK-mediated phosphorylation of RELA/p65, leading to activate expression of NF-kappa-B target genes. Associates with the I-kappa-B-kinase (IKK) core complex, composed of CHUK, IKBKB and IKBKG. Homodimer. Component of the shelterin complex (telosome) composed of TERF1, TERF2, TINF2, TERF2IP ACD and POT1. Interacts with TERF2; the interaction is direct. Does not interact with TERF1. Interacts with SLX4/BTBD12. Ubiquitous. Highly expressed. Belongs to the RAP1 family.

Protein type: DNA replication

Chromosomal Location of Human Ortholog: 16q23.1

Cellular Component: nucleoplasm; nuclear chromosome; chromosome, telomeric region; nuclear telomere cap complex; Mre11 complex; nuclear chromosome, telomeric region; cytoplasm; nuclear envelope; nucleus

Molecular Function: protein binding; telomeric DNA binding

Biological Process: negative regulation of telomere maintenance; positive regulation of I-kappaB kinase/NF-kappaB cascade; regulation of transcription, DNA-dependent; transcription, DNA-dependent; negative regulation of DNA recombination at telomere; telomere maintenance via telomerase; telomere maintenance; positive regulation of peptidyl-serine phosphorylation; activation of NF-kappaB transcription factor; protection from non-homologous end joining at telomere