Rabbit anti-Human SPINK5 Polyclonal Antibody | anti-SPINK5 antibody

SPINK5, NT (Serine Protease Inhibitor Kazal-type 5, Lympho-epithelial Kazal-type-related Inhibitor, LEKTI, Hemofiltrate Peptide HF6478, Hemofiltrate Peptide HF7665) (AP)

Cat. Num. AAA6500480

• Gene Names: SPINK5; NS; NETS; LEKTI; LETKI; VAKTI
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: SPINK5; Polyclonal Antibody; NT (Serine Protease Inhibitor Kazal-type 5; Lympho-epithelial Kazal-type-related Inhibitor; LEKTI; Hemofiltrate Peptide HF6478; Hemofiltrate Peptide HF7665) (AP); anti-SPINK5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SPINK5.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 1064

• Applicable Applications for anti-SPINK5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human SPINK5 (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of SPINK5 Antibody (N-term) in A2058 cell line lysates (35ug/lane). SPINK5 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(SPINK5 Antibody (N-term) (RB18954) IHC analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPINK5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(SPINK5 Antibody (N-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-SPINK5 antibody

Antibodies; Proteins

References

1. Nin, M., et.al., J. Dermatol. Sci. 54(1), 17-24 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 74027261
• NCBI GeneID: 11005
• NCBI Accession #: NP_006837
• NCBI GenBank Nucleotide #: NP_006837.2
• UniProt Accession #: Q9NQ38
• Molecular Weight: 120.714
• NCBI Official Full Name: serine protease inhibitor Kazal-type 5 isoform b preproprotein
• NCBI Official Synonym Full Names: serine peptidase inhibitor Kazal type 5
• NCBI Official Symbol: SPINK5
• NCBI Official Synonym Symbols: NS; NETS; LEKTI; LETKI; VAKTI
• NCBI Protein Information: serine protease inhibitor Kazal-type 5
• UniProt Protein Name: Serine protease inhibitor Kazal-type 5
• Protein Family: Serine protease inhibitor
• UniProt Gene Name: SPINK5
• UniProt Synonym Gene Names: LEKTI
• UniProt Entry Name: ISK5_HUMAN

NCBI Description

This gene encodes a multidomain serine protease inhibitor that contains 15 potential inhibitory domains. The encoded preproprotein is proteolytically processed to generate multiple protein products, which may exhibit unique activities and specificities. These proteins may play a role in skin and hair morphogenesis, as well as anti-inflammatory and antimicrobial protection of mucous epithelia. Mutations in this gene may result in Netherton syndrome, a disorder characterized by ichthyosis, defective cornification, and atopy. This gene is present in a gene cluster on chromosome 5. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]

Uniprot Description

SPINK5: Serine protease inhibitor, probably important for the anti-inflammatory and/or antimicrobial protection of mucous epithelia. Contribute to the integrity and protective barrier function of the skin by regulating the activity of defense- activating and desquamation-involved proteases. Inhibits KLK5, it's major target, in a pH-dependent manner. Inhibits KLK7, KLK14 CASP14, and trypsin. Defects in SPINK5 are the cause of Netherton syndrome (NETH). NETH is an autosomal recessive congenital ichthyosis associated with hair shaft abnormalities and anomalies of the immune system. Typical features are ichthyosis linearis circumflexa, ichthyosiform erythroderma, trichorrhexis invaginata (bamboo hair), atopic dermatitis, and hayfever. High postnatal mortality is due to failure to thrive, infections and hypernatremic dehydration. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Inhibitor; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; perinuclear region of cytoplasm; cytoplasm; extracellular region; cell cortex; cytosol

Molecular Function: serine-type endopeptidase inhibitor activity

Biological Process: extracellular matrix organization and biogenesis; negative regulation of angiogenesis; epithelial cell differentiation; negative regulation of immune response; epidermal cell differentiation; hair cell differentiation; regulation of T cell differentiation; anagen

Disease: Ige Responsiveness, Atopic; Netherton Syndrome