Rabbit anti-Human SPHK2 Polyclonal Antibody | anti-SPHK2 antibody

SPHK2 Antibody

Cat. Num. AAA9613554

• Gene Names: SPHK2; SK 2; SK-2; SPK 2; SPK-2
• Reactivity: Human
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Synonyms: SPHK2; Polyclonal Antibody; SPHK2 Antibody; SK 2; Sphingosine kinase 2; Sphingosine kinase type 2; SPHK2_HUMAN; SPK 2; anti-SPHK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: SPHK2 Antibody detects endogenous levels of total SPHK2.; Tissue Specificity: Mainly expressed in adult kidney, liver, and brain. Expressed in cerebral cortex and hippocampus (at protein level). Isoform 1 is the predominant form expressed in most tissues.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-SPHK2 antibody: Peptide ELISA (EIA)
• Application Notes: Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human SPHK2.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Phosphorylated by PKD on Ser-419 and Ser-421 upon PMA treatment. Phosphorylation induces export from the nucleus to the cytoplasm. Phosphorylated by MAPK1 and MAPK2 at Ser-387 and Thr-614, phosphorylation is induced by agonists such as EGF and PMA and increases kinase activity.Cleaved by CASP1 in apoptotic cells. The truncated form is released from cells.
• Subunit Structure: Interacts with histone H3. Interacts with HDAC1, HDAC2, MBD2 and SIN3A. Interacts with EEF1A1; the interaction enhances SPHK2 kinase activity. Interacts with PHB2 (By similarity).
• Subcellular Location: Cytoplasm. Nucleus. Endoplasmic reticulum. Mitochondrion inner membrane.; Note: In nucleus, located in nucleosomes where it associates with core histone proteins such as histone 3 (PubMed:19729656). In brains of patients with Alzheimer's disease, may be preferentially localized in the nucleus. Cytosolic expression decrease correlates with the density of amyloid deposits (PubMed:29615132). In apoptotic cells, colocalizes with CASP1 in cell membrane where is cleaved and released from cells in an active form (PubMed:20197547).Lysosome membrane.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Related Product Information for anti-SPHK2 antibody

Catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides. In contrast to prosurvival SPHK1, has a positive effect on intracellular ceramide levels, inhibits cells growth and enhances apoptosis. In mitochondria, is important for cytochrome-c oxidase assembly and mitochondrial respiration. The SPP produced in mitochondria binds PHB2 and modulates the regulation via PHB2 of complex IV assembly and respiration. In nucleus, plays a role in epigenetic regulation of gene expression. Interacts with HDAC1 and HDAC2 and, through SPP production, inhibits their enzymatic activity, preventing the removal of acetyl groups from lysine residues with histones. Upregulates acetylation of histone H3-K9, histone H4-K5 and histone H2B-K12. In nucleus, may have an inhibitory effect on DNA synthesis and cell cycle. In mast cells, is the main regulator of SPP production which mediates calcium influx, NF-kappa-B activation, cytokine production, such as TNF and IL6, and degranulation of mast cells (By similarity). In dopaminergic neurons, is involved in promoting mitochondrial functions regulating ATP and ROS levels (By similarity). Also involved in the regulation of glucose and lipid metabolism (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 323462187
• NCBI GeneID: 56848
• NCBI Accession #: NP_001191088.1
• NCBI GenBank Nucleotide #: NM_001204159.2
• UniProt Accession #: Q9NRA0; Q9BRN1; Q9H0Q2; Q9NWU7
• Molecular Weight: 69,217 Da
• NCBI Official Full Name: sphingosine kinase 2 isoform a
• NCBI Official Synonym Full Names: sphingosine kinase 2
• NCBI Official Symbol: SPHK2
• NCBI Official Synonym Symbols: SK 2; SK-2; SPK 2; SPK-2
• NCBI Protein Information: sphingosine kinase 2; sphingosine kinase type 2
• UniProt Protein Name: Sphingosine kinase 2
• Protein Family: Sphingosine kinase
• UniProt Gene Name: SPHK2
• UniProt Synonym Gene Names: SK 2; SPK 2
• UniProt Entry Name: SPHK2_HUMAN

NCBI Description

This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

SPHK2: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro- dihydrosphingosine, D-erythro-sphingosine and L-threo- dihydrosphingosine. Binds phosphoinositides. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - sphingolipid; EC 2.7.1.91; Kinase, lipid

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: membrane; lysosomal membrane; cytosol

Molecular Function: D-erythro-sphingosine kinase activity; protein binding; Ras GTPase binding; sphinganine kinase activity; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: blood vessel development; cell proliferation; sphingolipid metabolic process; sphingolipid biosynthetic process; positive regulation of cell proliferation; protein kinase C activation; sphingosine metabolic process; sphinganine-1-phosphate biosynthetic process; brain development; lipid phosphorylation; negative regulation of apoptosis